Evaluation of hydrophobic micro-zeolite-mixed matrix membrane and integrated with acetone-butanol-ethanol fermentation for enhanced butanol production.
Evaluation of hydrophobic micro-zeolite-mixed matrix membrane and integrated with acetone-butanol-ethanol fermentation for enhanced butanol production.
复制标题
疏水性微沸石混合基质膜与丙酮-丁醇-乙醇发酵相结合以提高丁醇产量的评估
DOI：
10.1186/s13068-015-0288-x
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发表时间：
2015
影响因子：
6.3
通讯作者：
Bai F
中科院分区：
文献类型：
--
作者：
Xue C;Yang D;Du G;Chen L;Ren J;Bai F
丁醇被认为是一种先进的生物燃料，可以从可再生生物质中提取。然而，微生物丁醇生产的主要挑战是丁醇滴度、收率和生产率较低，导致产品回收过程中能耗较高。各种替代分离技术，如萃取、吸附和气提等，可以与丙酮-丁醇-乙醇（ABE）发酵相结合，提高丁醇生产率，但它们的丁醇选择性并不令人满意。基于膜的渗透蒸发技术最近吸引了越来越多的关注，因为它具有潜在的理想的丁醇选择性。评估了沸石混合聚二甲基硅氧烷 (PDMS) 膜从丁醇/水二元溶液以及集成 ABE 发酵系统中的发酵液中回收丁醇的性能。 80°C 时，混合沸石的 PDMS 膜渗透液中的分离因子和丁醇滴度分别高达 33.0 和 334.6 g/L，随着膜中沸石负载量和进料温度的增加而增加。增强的丁醇分离因子归因于具有大孔径的疏水性沸石，提供了丁醇渗透的优选选择性路径。在结合全蒸发的分批补料发酵中，由 172.3 g/L 葡萄糖产生 54.9 g/L ABE（34.5 g/L 丁醇、17.0 g/L 丙酮和 3.4 g/L 乙醇）。总体丁醇生产率和收率分别增加了 16.0% 和 11.1%，这归因于 ABE 生产中酸的全蒸发和再同化减轻了丁醇抑制。沸石混合膜产生含有 169.6 g/L 丁醇或 253.3 g/L ABE 的高浓缩冷凝液，相分离后很容易得到含有 >600 g/L 丁醇的最终产品。 PDMS 基质中的沸石负载有助于改善膜的渗透蒸发性能，显示出回收高纯度丁醇的巨大潜力。因此，这种沸石混合 PDMS 膜与 ABE 发酵结合时具有提高生物丁醇产量的潜力。
Butanol is regarded as an advanced biofuel that can be derived from renewable biomass. However, the main challenge for microbial butanol production is low butanol titer, yield and productivity, leading to intensive energy consumption in product recovery. Various alternative separation technologies such as extraction, adsorption and gas stripping, etc., could be integrated with acetone–butanol–ethanol (ABE) fermentation with improving butanol productivity, but their butanol selectivities are not satisfactory. The membrane-based pervaporation technology is recently attracting increasing attention since it has potentially desirable butanol selectivity. The performance of the zeolite-mixed polydimethylsiloxane (PDMS) membranes were evaluated to recover butanol from butanol/water binary solution as well as fermentation broth in the integrated ABE fermentation system. The separation factor and butanol titer in permeate of the zeolite-mixed PDMS membrane were up to 33.0 and 334.6 g/L at 80°C, respectively, which increased with increasing zeolite loading weight in the membrane as well as feed temperature. The enhanced butanol separation factor was attributed to the hydrophobic zeolites with large pore size providing selective routes preferable for butanol permeation. In fed-batch fermentation incorporated with pervaporation, 54.9 g/L ABE (34.5 g/L butanol, 17.0 g/L acetone and 3.4 g/L ethanol) were produced from 172.3 g/L glucose. The overall butanol productivity and yield increased by 16.0 and 11.1%, respectively, which was attributed to the alleviated butanol inhibition by pervaporation and reassimilation of acids for ABE production. The zeolite-mixed membrane produced a highly concentrated condensate containing 169.6 g/L butanol or 253.3 g/L ABE, which after phase separation easily gave the final product containing >600 g/L butanol. Zeolite loading in the PDMS matrix was attributed to improving the pervaporative performance of the membrane, showing great potential to recover butanol with high purity. Therefore, this zeolite-mixed PDMS membrane had the potential to improve biobutanol production when integrating with ABE fermentation.