[Effect of precondition with GBE50 and Salviae miltionrrhizae on cycloxygenase-2 and its downstream effectors contents in ischemia/reperfusion myocardium].
[Effect of precondition with GBE50 and Salviae miltionrrhizae on cycloxygenase-2 and its downstream effectors contents in ischemia/reperfusion myocardium].
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发表时间：
2010-10
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通讯作者：
Y. Bao;Ai-hua Liu;Zhi-xiong Zhang
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作者：
Y. Bao;Ai-hua Liu;Zhi-xiong Zhang
目的观察大鼠心肌缺血再灌注（I/R）模型中环氧合酶2（考克斯2）及其下游效应物含量的变化，以及银杏叶提取物50（GBE 50）和丹参（SM）预处理对其的影响。方法采用结扎大鼠冠状动脉左前降支30 min，再灌注60 min的方法建立大鼠I/R模型。动物分为模型对照组、假手术组和实验组（造模前分别灌胃GBE 50和SM预处理1周）。实时荧光定量PCR检测心肌组织考克斯-2 mRNA的表达，放射免疫法检测血栓素B2（TXB 2）和6-酮-前列腺素F1 α（6-keto-PGF 1 α）的含量。结果模型组考克斯-2 mRNA表达明显高于假手术组（P < 0.001），各治疗组COX-2 mRNA表达明显下调（P < 0.01），TXB 2含量及TXB 2/PGF 1 α比值明显降低（P < 0.05）。此外，丹参还能上调心肌6-keto-PGF 1 α含量（P < 0.05）。结论I/R后考克斯-2通过血栓素A2和前列环素对心肌产生影响，GBE 50和SM均能抑制考克斯-2的产生，但作用途径不同。
OBJECTIVE To investigate the changes in contents of cycloxygenase-2 (COX-2) and its downstream effectors in rat's myocardial ischemia/reperfusion (I/R) model and observe the effects of precondition with GBE50 (Ginkgo biloba extract 50) and Salviae miltiorrhizae (SM) on them. METHODS Rat's I/R model was established by 30-min left anterior descending coronary artery occlusion followed with 60-min reperfusion. Animals were divided into the model control group, the sham-operated group and the tested groups (received 1-week precondition with GBE50 and SM respectively via intragastric infusion before modeling). COX-2 mRNA expression in myocardium was detected by real-time PCR; contents of thromboxane B2 (TXB2) and 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) were measured by radioimmunoassay. RESULTS The mRNA expression of COX-2 in the model group was obviously higher than that in the sham-operated group (P < 0.001), while that in the tested groups was down-regulated significantly (P < 0.01), and the content of TXB2 as well as the ratio of TXB2/PGF1alpha was reduced significantly (P < 0.05). Besides, SM also showed the up-regulation effect on 6-keto-PGF1alpha content in myocardium (P < 0.05). CONCLUSION COX-2 affects the myocardium through thromboxane A2 and prostacyclin after I/R; both GBE50 and SM can inhibit the production of COX-2, but they may act in different paths.