Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques
Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques
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DOI：
10.1093/nar/25.22.4692
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发表时间：
1997-11-15
影响因子：
14.9
通讯作者：
Martinez, I
中科院分区：
文献类型：
--
作者：
Aljanabi, SM;Martinez, I
本文介绍了一种简便、快速、通用、重复性好的从不同生物体中提取高质量的基因组DNA的方法。我们用同样的方法从不同组织（小麦、大麦、马铃薯、豆类、梨、杏仁叶以及真菌、昆虫、虾的新鲜组织）中提取了高质量的复杂基因组DNA。该方法不需要昂贵的和对环境有害的试剂和设备，甚至可以在低技术实验室中进行，该方法所需的组织量类似于50-100 mg，该方法提取的DNA的数量和质量足够高以进行数百个基于PCR的反应，并且还可以用于其他DNA操作技术，例如限制性消化，Southern杂交和克隆。
A very simple, fast, universally applicable and reproducible method to extract high quality megabase genomic DNA from different organisms is decribed, We applied the same method to extract high quality complex genomic DNA from different tissues (wheat, barley, potato, beans, pear and almond leaves as well as fungi, insects and shrimps' fresh tissue) without any modification. The method does not require expensive and environmentally hazardous reagents and equipment, It can be performed even in low technology laboratories, The amount of tissue required by this method is similar to 50-100 mg, The quantity and the quality of the DNA extracted by this method is high enough to perform hundreds of PCR-based reactions and also to be used in other DNA manipulation techniques such as restriction digestion, Southern blot and cloning.