A photoreceptor-specific cadherin is essential for the structural integrity of the outer segment and for photoreceptor survival
A photoreceptor-specific cadherin is essential for the structural integrity of the outer segment and for photoreceptor survival
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DOI：
10.1016/s0896-6273(01)00531-1
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发表时间：
2001-12-06
期刊：
影响因子：
16.2
通讯作者：
Nathans, J
中科院分区：
文献类型：
--
作者：
Rattner, A;Smallwood, PM;Nathans, J
通过消减杂交和高通量测序在视网膜 cDNA 中鉴定出钙粘蛋白家族成员 prCAD。 prCAD 仅在视网膜感光细胞中表达，并且 prCAD 蛋白定位于视杆细胞和视锥细胞外节的基部。在 prCAD(-/-) 小鼠中，外部节段杂乱且破碎，并且感光细胞进行性死亡。 prCAD 不太可能参与内节和外节之间的蛋白质运输，因为在 prCAD(-/-) 小鼠中，光转导蛋白似乎已正确定位，并且视杆细胞和视锥细胞的光反应仅受到适度损害。这些实验意味着 prCAD 具有高度专门化的细胞生物学功能，并表明局部粘附活性对于外节完整性至关重要。
A cadherin family member, prCAD, was identified in retina cDNA by subtractive hybridization and high throughput sequencing. prCAD is expressed only in retinal photoreceptors, and the prCAD protein is localized to the base of the outer segment of both rods and cones. In prCAD(-/-) mice, outer segments are disorganized and fragmented, and there is progressive death of photoreceptor cells. prCAD is unlikely to be involved in protein trafficking between inner and outer segments, since phototransduction proteins appear to be correctly localized and the light responses of both rods and cones are only modestly compromised in prCAD(-/-) mice. These experiments imply a highly specialized cell biological function for prCAD and suggest that localized adhesion activity is essential for outer segment integrity.