Effect of time of administration of an inflammatory agent on plasma corticosterone and haptoglobin levels in the rat.
Effect of time of administration of an inflammatory agent on plasma corticosterone and haptoglobin levels in the rat.
复制标题
炎症剂给药时间对大鼠血浆皮质酮和触珠蛋白水平的影响。
作者：
A. Szafarczyk;J. Moretti;J. Boissin;I. Assenmacher
为了研究引起急性炎症反应的时钟时间对皮质酮和结合珠蛋白高分泌动力学的影响，给雌性大鼠皮下注射松节油(0.5ml⁄100g体重)，在12小时光照-12小时黑暗条件下分别于09小时、13小时、21小时和03小时给予松节油。在每个实验中，15组5只动物在注射后10分钟至120小时的时间间隔(a.i.)被处死。血浆样本用改进的荧光法测定皮质酮(C)，结合珠蛋白-血红蛋白复合体的过氧化活性测定结合珠蛋白(HP)(Jayle，1951)。C的高分泌在4组均呈双时相型。第一，应激性C升高在所有组均达到相同的上限水平(约100微克⁄100ml)。然而，人工智能的时间滞后。C峰的变化范围为20min。在注射15小时或21小时的大鼠中，到03小时应激动物中的1~2小时。第二个持久的C增强阶段..。
In order to examine the influence of the clock hour at which an acute inflammatory reaction is induced on the kinetics of both corticosterone and haptoglobin hyperproduction, turpentine oil was administered sc to female rats (0.5 ml⁄100 g body wt), kept under a 12 hr light- 12 hr dark light regimen, at 09 hr, 13 hr, 21 hr and 03 hr. In each experiment 15 groups of 5 animals were sacrificed at time intervals ranging from 10 min to 120 hr after injections (a.i.). The plasma samples were assayed for corticosterone (C) by an improved fluorometric method, and for haptoglobin (Hp) by the peroxidatic activity of a haptoglobin-hemoglobin complex (Jayle, 1951). The C hypersecretion exhibited in the 4 groups a biphasic pattern. The first, stressinduced C increase attained in all groups the same ceiling level (about 100 µg⁄100 ml). However the time lag a.i. of the C peak varied from 20 min. in the 15 hr or 21 hr-injected rats, to 1 to 2 hr in the 03-hr stressed animals. The secondary, long-lasting C augmentation sta...