Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes
Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes
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DOI：
10.1016/j.snb.2010.07.021
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发表时间：
2010-09-21
影响因子：
8.4
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Sasaki, Hirotaka;Onoe, Hiroaki;Takeuchi, Shoji
荧光分子的吸收是用PDMS制造的微荧光器件的一个棘手的性质。吸收提高了来自PDMS的荧光背景水平，并降低了注入PDMS通道中的荧光分子的浓度。在PDMS表面上的聚对二甲苯基本上抑制了罗丹明B的吸收在该技术的测试中，我们发现聚对二甲苯涂覆的PDMS通道可用于使用荧光强度的精确分析（C）2010 Elsevier B V保留所有权利
Absorption of fluorescent molecules is a troublesome property of microfluridic devices fabricated with PDMS The absorption raises background-levels of fluorescence from PDMS, and reduces concentrations of injected fluorescent molecules in PDMS channels We report here that conformal deposition of polyp-xylylene derivatives (parylenes) on the surface of PDMS substantially suppressed the absorption of Rhodamine B In a test of this technique, we found parylene-coated PDMS channels to be useful in accurate analyses using fluorescence intensity (C) 2010 Elsevier B V All rights reserved