Role of Phosphodiesterase2A in Proliferation and Migration of Human Osteosarcoma Cells
Role of Phosphodiesterase2A in Proliferation and Migration of Human Osteosarcoma Cells
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DOI：
10.21873/anticanres.13812
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发表时间：
2019-11-01
影响因子：
2
通讯作者：
Arai, Naoya
中科院分区：
文献类型：
--
作者：
Murata, Taku;Shimizu, Kasumi;Arai, Naoya
背景/目的：骨肉瘤患者的预后差，因此，迫切需要新的治疗策略。磷酸二酯酶2（PDE 2）是磷酸二酯酶超家族的11个家族（PDE 1-PDE 11）之一，其调节细胞内cAMP和cGMP的浓度和作用。本体外研究旨在研究PDE 2在人口腔骨肉瘤HOSM-1细胞中的作用。材料和方法：通过cAMP-PDE测定和实时-PCR测量PDE 2表达。评估PDE 2特异性抑制剂，异丙基-9-（2-羟基-3-壬基）腺嘌呤（EHNA）、8-溴-cAMP和8-溴-cGMP对细胞增殖和迁移的影响。结果：HOSM-1细胞中存在PDE 2活性和PDE 2A mRNA表达。EHNA和8-bromo-cAMP对细胞增殖有抑制作用，而8-bromo-cGMP对细胞增殖无抑制作用。EHNA和8-bromo-cGMP可促进细胞迁移，而8-bromo-cAMP则可抑制细胞迁移。结论：HOSM-1细胞增殖受PDE 2-cAMP信号调控，细胞迁移受PDE 2-cGMP信号调控。
Background/Aim: The prognosis of patients with osteosarcoma is poor; therefore, new treatment strategies are urgently needed. Phosphodiesterase 2 (PDE2) is one of the 11 families (PDE1-PDE11) of the phosphodiesterase superfamily that regulates the intracellular concentrations and effects of cAMP and cGMP. This in vitro study was performed to investigate the role of PDE2 in human oral osteosarcoma HOSM-1 cells. Materials and Methods: PDE2 expression was measured by a cAMP-PDE assay and real-time-PCR. The effects of the PDE2-specific inhibitors, erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), 8-bromo-cAMP, and 8-bromo-cGMP on cell proliferation and migration were assessed. Results: PDE2 activity and PDE2A mRNA expression were detected in HOSM-1 cells. Cell proliferation was inhibited by EHNA and 8-bromo-cAMP but not by 8-bromo-cGMP. Cell migration was stimulated by EHNA and 8-bromo-cGMP, but it was inhibited by 8-bromo-cAMP. Conclusion: Cell proliferation is regulated by PDE2-cAMP signaling and cell migration is regulated by PDE2-cGMP signaling in HOSM-1 cells.