Biodegradable PCL scaffolds with an interconnected spherical pore network for tissue engineering
Biodegradable PCL scaffolds with an interconnected spherical pore network for tissue engineering
复制标题
DOI：
10.1002/jbm.a.31396
复制
发表时间：
2008-04-01
影响因子：
4.9
通讯作者：
Suay, J.
中科院分区：
文献类型：
--
作者：
Izquierdo, R.;Garcia-Giralt, N.;Suay, J.
本文介绍了一种用于组织工程支架的可控互联多孔结构的制备技术。该技术的基础是制备互连的聚甲基丙烯酸乙酯(PEMA)微球模板，引入可生物降解的聚合物聚F-己内酯(PCL)，并通过选择性溶剂消除模板。制备了孔隙率为70%、孔径达200 pm的一系列PCL支架，并对其进行了热学和力学性能表征。将人软骨细胞单层培养在块状PCL盘上或种植到多孔PCL支架上。检测细胞黏附、活性、增殖和蛋白多糖(PG)的合成，并与单层培养进行比较，以组织处理的聚苯乙烯或颗粒培养为对照。在PCL盘上培养的细胞显示出类似于聚苯乙烯对照组的粘附性(允许高水平的增殖)。染色的支架切片可见圆形软骨细胞聚集体镶嵌在多孔的PCL内。PG产量与颗粒培养相似，高于单层融合后培养。这表明细胞能够将自己附着在PCL上。此外，在多孔PCL中，细胞保持与天然软骨内软骨细胞相同的表型。这些结果表明，PCL支架可能是一种合适的软骨细胞培养材料。(C)2007年威利期刊公司。
A technique for producing controlled interconnected porous structures for application as a tissue engineering scaffold is presented in this article. The technique is based on the fabrication of a template of interconnected poly(ethyl methacrylate) (PEMA) microspheres, the introduction of a biodegradable polymer, poly-F-caprolactone (PCL), and the elimination of the template by a selective solvent. A series of PCL scaffolds with a porosity of 70% and pore sizes up to 200 pm were produced and characterized (both thermally and mechanically). Human chondrocytes were cultured in monolayer on bulk PCL disks or seeded into porous PCL scaffolds. Cell adhesion, viability, proliferation, and proteoglycan (PG) synthesis were tested and compared with monolayer cultures on tissue-treated polystyrene or pellet cultures as reference controls. Cells cultured on PCL disks showed an adhesion similar to that of the polystyrene control (which allowed high levels of proliferation). Stained scaffold sections showed round-shaped chondrocyte aggregates embedded into porous PCL. PG production was similar to that of the pellet cultures and higher than that obtained with monolayer postconfluence cultures. This shows that the cells are capable of attaching themselves to PCL. Furthermore, in porous PCL, cells maintain the same phenotype as the chondrocytes within the native cartilage. These results suggest that PCL scaffolds may be a suitable candidate for chondrocyte culture. (c) 2007 Wiley Periodicals, Inc.