Analyzing Molecular Basis of Heat-Induced Loss-of-Wheat Resistance to Hessian Fly (Diptera: Cecidomyiidae) Infestation Using RNA-Sequencing
Analyzing Molecular Basis of Heat-Induced Loss-of-Wheat Resistance to Hessian Fly (Diptera: Cecidomyiidae) Infestation Using RNA-Sequencing
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DOI：
10.1093/jee/toaa058
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发表时间：
2020-04
影响因子：
2.2
通讯作者：
Lieceng Zhu;Jiazheng (John) Yuan;J. O'neal;Daria Brown;Ming-shun Chen
中科院分区：
文献类型：
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作者：
Lieceng Zhu;Jiazheng (John) Yuan;J. O'neal;Daria Brown;Ming-shun Chen
摘要高温胁迫降低了小麦对麦蛾（HF，Mayetiola destructor（Say））（双翅目：Cecidomyiidae）侵染的抗性。本研究的目的是利用RNA测序（RNA-seq）技术分析热诱导小麦对HF侵染抗性丧失的分子基础。为此，两个抗小麦品种'Molly'和'考德威尔'分别含有抗性基因H13和H6，感染与无毒HF生物型GP和处理不同的温度，以检查热胁迫对其抗性表型的影响。对从Molly植物中的HF饲喂位点收集的组织样品进行RNA-seq分析，以确定热应激对小麦植物中基因的转录物表达的影响。结果表明，考德威尔对HF的抗性比Molly对热胁迫更敏感，热胁迫下调了木质素和角质层蜡质的初级代谢和生物合成相关基因，上调了生长素和12-氧代-植物二烯酸（OPDA）信号通路相关基因。我们的研究结果和以前的报告表明，热胁迫可能会损害过程中产生和调动化学资源所需的合成防御化合物，削弱细胞壁和角质层防御，减少OPDA信号，但增加生长素信号，导致抑制抗性和敏感性的激活。
Abstract Heat stress compromises wheat resistance to Hessian fly (HF, Mayetiola destructor (Say)) (Diptera: Cecidomyiidae) infestation. The objective of this research is to analyze the molecular basis of heat-induced loss of wheat resistance to HF infestation using RNA Sequencing (RNA-seq). To this end, two resistant wheat cultivars ‘Molly' and ‘Caldwell’ containing the resistance genes H13 and H6, respectively, were infested with an avirulent HF biotype GP and treated with different temperatures to examine the impact of heat stress on their resistance phenotypes. Tissue samples collected from HF feeding sites in Molly plants were subjected to RNA-seq analysis to determine the effect of heat stress on transcript expression of genes in wheat plants. Our results indicate that resistance to HF infestation in Caldwell is more sensitive to heat stress than that in Molly, and that heat stress down-regulates most genes involved in primary metabolism and biosynthesis of lignin and cuticular wax, but up-regulate most or all genes involved in auxin and 12-oxo-phytodienoic acid (OPDA) signaling pathways. Our results and previous reports suggest that heat stress may impair the processes in wheat plants that produce and mobilize chemical resources needed for synthesizing defensive compounds, weaken cell wall and cuticle defense, decrease OPDA signaling, but increase auxin signaling, leading to the suppressed resistance and activation of susceptibility.