Influence of reaction conditions oil the oxidative behavior of model Maillard-reaction products
Influence of reaction conditions oil the oxidative behavior of model Maillard-reaction products
复制标题
DOI：
10.1021/jf970040v
复制
发表时间：
1997-12-01
影响因子：
6.1
通讯作者：
Kitts, DD
中科院分区：
文献类型：
--
作者：
Wijewickreme, AN;Kitts, DD
通过D-葡萄糖或D-果糖与L赖氨酸在四种不同的初始反应参数(pH、水分活度、反应时间和温度)下反应，合成了模型非透析性美拉德反应产物(MRPs)，并对其潜在的抗氧化剂或促氧化剂活性和遗传毒性进行了评价。在以硫代巴比妥酸(TBA)作为脂质氧化终点的无金属模型亚油酸乳液体系中，所有模型Glu-Lys和Fru-Lys MRP都显示出不同程度的抗氧化活性。相同的MRPs在铜离子存在下由氧电极测定的氧化行为包括对特定MRPs的促氧化和抗氧化活性。利用体外DNA模型系统，Glu-Lys和Fru-Lys MRP混合物在高浓度(>；0.001%，w/v)时均可引起PM2噬菌体DNA链的显著断裂。总体而言，与在相同条件下合成的Glu-Lys MRPs相比，Fru-Lys MRPs具有更强的促氧化和遗传毒性活性。结果表明，使用一些不同的体外模型系统来定义在特定不同条件下合成的不同MRP的特征氧化行为是重要的。
Model nondialyzable Maillard reaction products (MRPs) were synthesized by reacting D-glucose or D-fructose with L-lysine under four different initial reaction parameters (pH, water activity, reaction time, and temperature) and evaluated for potential antioxidant or prooxidant activity and genotoxic properties. All model Glu-Lys and Fru-Lys MRPs showed various degrees of antioxidant activity when evaluated in a metal-free model linoleic acid emulsion system using thiobarbituric acid (TBA) as an end point measure of lipid oxidation. The oxidative behavior of the same MRPs, when determined by an oxygen electrode in the presence of copper ions, consisted of both prooxidant and antioxidant activities for specific MRPs. Using an in vitro DNA model system, both Glu-Lys and Fru-Lys MRP mixtures induced significant (p less than or equal to 0.05) strand breakage in PM2 bacteriophage DNA at high concentrations (>0.001%, w/v). In general, Fru-Lys MRPs exhibited more prooxidant and genotoxic activity compared to Glu-Lys MRPs synthesized under identical conditions. The results demonstrate the importance of using a number of different in vitro model systems in defining the characteristic oxidative behavior of different MRPs synthesized under specifically different conditions.