Smart Cup: A Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostic Device.
Smart Cup: A Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostic Device.
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DOI：
10.1016/j.snb.2016.01.073
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发表时间：
2016-06-28
期刊：
影响因子：
--
通讯作者：
Liu C
中科院分区：
文献类型：
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作者：
Liao SC;Peng J;Mauk MG;Awasthi S;Song J;Friedman H;Bau HH;Liu C
基于核酸扩增的诊断为检测和监测传染病的进展提供了快速、灵敏和特异的手段。然而，这种方法通常需要大量的样品制备、昂贵的仪器和训练有素的人员。所有这些都阻碍了在资源有限的环境中使用这种方法，因为在这些环境中，许多传染病是地方病。在这里，我们报告了一种简单，廉价，最小仪器化的智能杯平台，用于在护理点对病原体进行快速，定量的分子诊断。我们的智能杯利用水引发的放热化学反应为基于核酸的等温扩增提供热量。放大温度通过相变材料（PCM）调节。当环境温度范围为12至35°C时，PCM将扩增反应器保持在恒定温度，通常为60-65°C。为了消除对光学检测器的需求并最大限度地降低成本，我们使用智能手机的闪光灯来激发荧光染料，并使用手机摄像头来记录放大过程中的实时荧光发射。智能手机可以同时监控多个扩增反应器并分析记录的数据。我们的智能杯的实用性证明了扩增和定量单纯疱疹病毒2型（HSV-2）与LAMP检测在我们定制的微流控诊断芯片。我们始终在每个样本中检测到少至100个拷贝的HSV-2病毒DNA。我们的系统不需要任何实验室设施，适用于家庭、现场和诊所，以及资源匮乏的环境，在这些环境中，使用先进的实验室是不切实际的、负担不起的或根本不存在的。
Nucleic acid amplification-based diagnostics offer rapid, sensitive, and specific means for detecting and monitoring the progression of infectious diseases. However, this method typically requires extensive sample preparation, expensive instruments, and trained personnel. All of which hinder its use in resource-limited settings, where many infectious diseases are endemic. Here, we report on a simple, inexpensive, minimally-instrumented, smart cup platform for rapid, quantitative molecular diagnostics of pathogens at the point of care. Our smart cup takes advantage of water-triggered, exothermic chemical reaction to supply heat for the nucleic acid-based, isothermal amplification. The amplification temperature is regulated with a phase-change material (PCM). The PCM maintains the amplification reactor at a constant temperature, typically, 60-65°C, when ambient temperatures range from 12 to 35°C. To eliminate the need for an optical detector and minimize cost, we use the smartphone’s flashlight to excite the fluorescent dye and the phone camera to record real-time fluorescence emission during the amplification process. The smartphone can concurrently monitor multiple amplification reactors and analyze the recorded data. Our smart cup’s utility was demonstrated by amplifying and quantifying herpes simplex virus type 2 (HSV-2) with LAMP assay in our custom-made microfluidic diagnostic chip. We have consistently detected as few as 100 copies of HSV-2 viral DNA per sample. Our system does not require any lab facilities and is suitable for use at home, in the field, and in the clinic, as well as in resource-poor settings, where access to sophisticated laboratories is impractical, unaffordable, or nonexistent.