Spiral intercellular calcium waves in hippocampal slice cultures
Spiral intercellular calcium waves in hippocampal slice cultures
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DOI：
10.1152/jn.1998.79.2.1045
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发表时间：
1998-02-01
影响因子：
2.5
通讯作者：
Charles, AC
中科院分区：
文献类型：
--
作者：
Harris-White, ME;Zanotti, SA;Charles, AC
新生小鼠海马切片器官型培养物的 CA1 和 CA2 区域存在复杂的细胞间钙信号传导模式。涉及 5-15 个细胞的自发局部细胞间 Ca2+ 波从层 Oriens 和 s 中的多个焦点同心传播。辐射。在这些相同区域，涉及数百个细胞的广泛 Ca2+ 波以曲线和螺旋波前的形式传播到 CA1 和 CA2 的广阔区域。 Ca2+波以5-10μm/s的速率传播，被毒胡萝卜素消除，并且不需要细胞外Ca2+。星形胶质细胞和神经元的染色表明这些细胞间波主要发生在星形胶质细胞中。 Ca2+ 波的频率和振幅响应 N-甲基-D-天冬氨酸 (NMDA) 浴的应用而增加，并响应细胞外 Ca2+ 的去除或河豚毒素的应用而降低。这种细胞间 Ca2+ 信号传导的新模式是可兴奋介质行为的特征。海马体中的神经胶质细胞网络可能充当可兴奋介质，其空间和时间信号传导特性受到神经元活动的调节。
Complex patterns of intercellular calcium signaling occur in the CA1 and CA2 regions of hippocampal slice organotypic cultures from neonatal mice. Spontaneous localized intercellular Ca2+ waves involving 5-15 cells propagate concentrically from multiple foci in the stratum oriens and s. radiatum. In these same regions, extensive Ca2+ waves involving hundreds of cells propagate as curvilinear and spiral wavefronts across broad areas of CA1 and CA2. Ca2+ waves travel at rates of 5-10 mu m/s, are abolished by thapsigargin, and do not require extracellular Ca2+. Staining for astrocytes and neurons indicates that these intercellular waves occur primarily in astrocytes. The frequency and amplitude of Ca2+ waves increase in response to bath application of N-methyl-D-aspartate (NMDA) and decrease in response to removal of extra-cellular Ca2+ or application of tetrodotoxin. This novel pattern of intercellular Ca2+ signaling is characteristic of the behavior of an excitable medium. Networks of glial cells in the hippocampus may behave as an excitable medium whose spatial and temporal signaling properties are modulated by neuronal activity.