Follicle-stimulating hormone-dependent phosphorylation of vimentin in cultures of rat Sertoli cells.
Follicle-stimulating hormone-dependent phosphorylation of vimentin in cultures of rat Sertoli cells.
复制标题
大鼠支持细胞培养物中波形蛋白的卵泡刺激激素依赖性磷酸化。
DOI：
10.1073/pnas.80.4.993
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发表时间：
1983
影响因子：
11.1
通讯作者：
Kierszenbaum,AL
中科院分区：
文献类型：
--
作者：
Spruill,WA;Steiner,AL;Tres,LL;Kierszenbaum,AL
内源性蛋白磷酸化的研究在培养的大鼠睾丸支持细胞处理后，卵泡刺激素（FSH）和药物激活cAMP依赖性蛋白激酶。在完整的支持细胞中，磷酸化和去磷酸化的蛋白质发生在这些药物治疗的反应。使用无细胞制剂的研究表明，在完整细胞中，cAMP或cAMP依赖性蛋白激酶的催化亚基磷酸化的四种磷蛋白也以FSH依赖性方式磷酸化。这些数据表明，支持细胞中FSH依赖性磷酸化通过cAMP依赖性蛋白激酶的激活而发生。一种分子量为58，000的FSH依赖性磷蛋白被鉴定为中间丝蛋白波形蛋白，基于其在二维凝胶中的迁移及其肽图。用FSH处理后，通过免疫荧光法监测支持细胞中波形蛋白的细胞分布。本研究的结果支持中间丝在支持细胞FSH依赖性事件中的作用。
Endogenous protein phosphorylation was investigated in cultured rat Sertoli cells after treatment with follicle-stimulating hormone (FSH) and pharmacological agents that activate cAMP-dependent protein kinases. In intact Sertoli cells, both phosphorylation and dephosphorylation of proteins occurred in response to treatment with these agents. Studies using cell-free preparations suggest that four phosphoproteins phosphorylated by cAMP or the catalytic subunit of cAMP-dependent protein kinase were also phosphorylated in a FSH-dependent manner in intact cells. These data suggest that FSH-dependent phosphorylation in Sertoli cells occurs through activation of a cAMP-dependent protein kinase. A FSH-dependent phosphoprotein with a molecular weight of 58,000 was identified as the intermediate filament protein vimentin, based on its migration in two-dimensional gels and its peptide map. The cellular distribution of vimentin was monitored by immunofluorescence in Sertoli cells after treatment with FSH. Results of this study support a role for intermediate filaments in FSH-dependent events in Sertoli cells.