Grape Seed Proanthocyanidin Extract Ameliorates Cardiac Remodelling After Myocardial Infarction Through PI3K/AKT Pathway in Mice.
Grape Seed Proanthocyanidin Extract Ameliorates Cardiac Remodelling After Myocardial Infarction Through PI3K/AKT Pathway in Mice.
复制标题
葡萄籽原花青素提取物通过 PI3K/AKT 通路改善小鼠心肌梗死后的心脏重塑
DOI：
10.3389/fphar.2020.585984
复制
发表时间：
2020
影响因子：
5.6
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Ruan Y;Jin Q;Zeng J;Ren F;Xie Z;Ji K;Wu L;Wu J;Li L
心肌梗死是全球最严重的致死性疾病之一，它是由冠状动脉急性闭塞引起的。葡萄籽原花青素提取物（GSPE）是从葡萄籽中提取的具有抗氧化、抗炎和抗肿瘤药理作用的活性化合物。天然产品价格便宜，容易获得，应用广泛，效果显著。它已被用于治疗许多疾病，如癌症，脑损伤和糖尿病并发症。然而，关于其在小鼠心肌梗死中的作用及其相关机制的研究有限。本研究表明，GSPE治疗小鼠可显著减轻心功能障碍，改善MI损伤所致的病理变化。GSPE可抑制H9 C2细胞缺氧后的凋亡，使Bax表达降低，Bcl-2表达升高。在体内外MI模型中均检测到p-PI 3 K和p-AKT的高表达。特异性PI 3 K/AKT通路抑制剂LY 294002的使用使GSPE的心脏保护作用消退。结果表明，GSPE可通过PI 3 K/AKT信号通路改善心肌梗死后心功能不全和重构，抑制缺氧状态下心肌细胞凋亡。
Myocardial infarction is one of the most serious fatal diseases in the world, which is due to acute occlusion of coronary arteries. Grape seed proanthocyanidin extract (GSPE) is an active compound extracted from grape seeds that has anti-oxidative, anti-inflammatory and anti-tumor pharmacological effects. Natural products are cheap, easy to obtain, widely used and effective. It has been used to treat numerous diseases, such as cancer, brain injury and diabetes complications. However, there are limited studies on its role and associated mechanisms in myocardial infarction in mice. This study showed that GSPE treatment in mice significantly reduced cardiac dysfunction and improved the pathological changes due to MI injury. In vitro, GSPE inhibited the apoptosis of H9C2 cells after hypoxia culture, resulting in the expression of Bax decreased and the expression of Bcl-2 increased. The high expression of p-PI3K and p-AKT was detected in MI model in vivo and in vitro. The use of the specific PI3K/AKT pathway inhibitor LY294002 regressed the cardio-protection of GSPE. Our results showed that GSPE could improve the cardiac dysfunction and remodeling induced by MI and inhibit cardiomyocytes apoptosis in hypoxic conditions through the PI3K/AKT signaling pathway.
登录
查看更多内容
影响因子：
3.4
作者：
Gao Z;Liu G;Hu Z;Shi W;Chen B;Zou P;Li X
通讯作者：
Li X
DOI：
10.1186/s12964-014-0071-9
发表时间：
2014-11-14
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Li H;Dusseault J;Larose L
通讯作者：
Larose L
褪黑激素通过 PI3K/Akt 依赖性机制减轻脓毒症引起的心脏功能障碍
影响因子：
9.5
作者：
An, Rui;Zhao, Lei;Sun, Lijun
通讯作者：
Sun, Lijun
影响因子：
4.8
作者：
Ghosh, Samrat;Bishayee, Kausik;Khuda-Bukhsh, Anisur Rahman
通讯作者：
Khuda-Bukhsh, Anisur Rahman
影响因子：
5
作者：
Mocanu, MM;Bell, RM;Yellon, DM
通讯作者：
Yellon, DM