Expression of BDNF and trkB as a function of age and cognitive performance
Expression of BDNF and trkB as a function of age and cognitive performance
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DOI：
10.1016/s0006-8993(98)00993-7
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发表时间：
1998-11-23
期刊：
影响因子：
2.9
通讯作者：
Wiegand, SJ
中科院分区：
文献类型：
--
作者：
Croll, SD;Ip, NY;Wiegand, SJ
神经营养因子BDNF的表达在阿尔茨海默病患者的学习相关事件中增加，而在海马体中减少，这表明它在学习、记忆和/或与年龄相关的记忆缺陷中起作用。我们检测了BDNF及其高亲和力受体trkB在年轻和老年Sprague-Dawley大鼠中的表达。半定量原位杂交法检测BDNF和trkB mRNA， ELISA法检测BDNF蛋白。随着年龄的增长，笔尖中BDNF mRNA、中脑中BDNF蛋白和大脑许多区域的trkB mRNA均显著下降。大鼠献祭前进行Morris水迷宫评估，BDNF和trkB水平与认知状态有关。回归分析显示，脑桥trkB mRNA的减少显著预测了老年大鼠的记忆能力受损。这些结果表明，trkB mRNA随年龄的下降比BDNF的下降更为普遍，并且BDNF的下降仅限于更多的脑尾侧区域。(C) 1998 Elsevier Science B.V.版权所有
The expression of the neurotrophic factor BDNF increases during learning-related events and is decreased in the hippocampus of Alzheimer's Disease patients, suggesting that it plays a role in learning, memory, and/or age-related memory deficits. We examined the expression of BDNF and its high affinity receptor, trkB, in young and aged Sprague-Dawley rats. BDNF and trkB mRNA were measured by semi-quantitative in situ hybridization and BDNF protein was measured by ELISA. Significant decreases with age were detected for BDNF mRNA in the pens, BDNF protein in the midbrain, and trkB mRNA in many areas of the brain. Rats were evaluated on the Morris water maze before sacrifice so that BDNF and trkB levels could be related to cognitive status. Regression analyses revealed that decreased trkB mRNA in the pons significantly predicted impaired memory performance in aged rats. These results suggest that decreases in trkB mRNA with age are more widespread than decreases in BDNF, and that BDNF decreases are restricted to more caudal brain regions. (C) 1998 Elsevier Science B.V. All rights reserved.