BCMAP - AN INTEGRAL MEMBRANE-PROTEIN IN THE GOLGI-APPARATUS OF HUMAN MATURE B-LYMPHOCYTES
BCMAP - AN INTEGRAL MEMBRANE-PROTEIN IN THE GOLGI-APPARATUS OF HUMAN MATURE B-LYMPHOCYTES
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DOI：
10.1093/intimm/7.7.1093
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发表时间：
1995-07-01
影响因子：
4.4
通讯作者：
TSAPIS, A
中科院分区：
文献类型：
--
作者：
GRAS, MP;LAABI, Y;TSAPIS, A
BCMA 是一种人类基因，优先在成熟淋巴细胞中表达为 1.2 kb mRNA，编码 184 个氨基酸肽 (BCMAp)。使用具有淋巴细胞分化不同阶段特征的人恶性a细胞系对BCMA mRNA表达进行研究，证明BCMA mRNA在前B淋巴细胞阶段不存在。它在前 B 细胞阶段表达微弱，并且随着淋巴细胞的成熟而表达增加。使用多克隆抗体，通过细胞分级分离和免疫沉淀表明 BCMAp 是一种非糖基化的整合膜蛋白。此外，BCMAp 在体外作为 I 型整合膜蛋白插入犬微粒体中。细胞表面标记显示BCMAp在成熟a淋巴细胞的质膜中不表达。免疫荧 subcompartments强烈表明BCMAp位于高尔基体的cia部分，因此，BCMAp是第一个具有组织特异性的高尔基驻留蛋白，其表达与淋巴细胞的分化阶段相关。 BCMAp 在高尔基体中的位置及其在浆细胞（分泌大量 Ig）中的高表达表明 BCMAp 与 Ig 的细胞内运输有关。
BCMA is a human gene expressed preferentially in mature a lymphocytes as a 1.2 kb mRNA, which encodes a 184 amino acid peptide (BCMAp). The study of BCMA mRNA expression, using human malignant a cell lines characteristic of different stages of a lymphocyte differentiation, demonstrated that the BCMA mRNA is absent in the pro-B lymphocyte stage. It is expressed faintly at the pre-B cell stage and its expression increases with a lymphocyte maturation. Polyclonal antibodies were used to show, by cellular fractionation and immunoprecipitation, that BCMAp is a non-glycosylated integral membrane protein. Furthermore, BCMAp inserts, in vitro, into canine microsomes, as a type I integral membrane protein. Cell surface labeling showed that BCMAp is not expressed in the plasma membrane of mature a lymphocytes. Immunofluorescence studies revealed that BCMAp lies in a cap-like structure near the nucleus, that was identified as the Golgi apparatus by co-localization of BCMAp with CTR433, a marker of the medial cisternae of the Golgi apparatus, Confocal scanning laser microscopy of U266 plasma cells labeled with markers of various Golgi apparatus subcompartments strongly suggests that BCMAp is located in the cia part of the Golgi apparatus, Thus, BCMAp is the first Golgi resident protein with a tissue specificity and whose expression is linked to the stage of differentiation of a lymphocytes. The location of BCMAp in the Golgi apparatus and its high expression in plasmocytes (secreting large amounts of Ig) suggest that BCMAp is implicated in the intracellular traffic of Ig.