Strain-related differences after experimental traumatic brain injury in rats.
Strain-related differences after experimental traumatic brain injury in rats.
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大鼠实验性脑外伤后应变相关的差异。
DOI：
10.1089/neu.2010.1270
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发表时间：
2010
影响因子：
4.2
通讯作者：
Sun,Dong
中科院分区：
文献类型：
--
作者：
Reid,WendyMurdock;Rolfe,Andrew;Register,David;Levasseur,JosephE;Churn,SevernB;Sun,Dong
本研究直接比较了两种常用大鼠品系（Fisher 344和Sprague-Dawley）实验性脑损伤的影响。我们以前发现，费舍尔大鼠有更高的死亡率和更频繁的癫痫发作在相同的损伤水平比Sprague-Dawley大鼠。虽然大鼠的应变差异通常被认为是导致研究变异性的原因，但涉及实验性神经创伤中应变影响的文献很少。因此，本研究比较了两种大鼠品系在侧向液压冲击损伤后的结果。监测Fisher 344和Sprague-Dawley大鼠的生理测量、颅内压和脑电图活动的变化。我们使用Fluoro-Jade-B（FJB）组织化学和caspase-3免疫染色进一步分析了损伤脑中的神经元变性和细胞死亡。行为学研究使用梁行走和Morris水迷宫进行，以表征损伤后运动和认知功能恢复的应变差异。我们发现，Fisher大鼠有显着更高的颅内压，延长癫痫发作的活动，增加FJB阳性染色在受损的皮质和丘脑，并增加caspase-3的表达比Sprague-Dawley大鼠。平均而言，Fisher大鼠在癫痫发作活动中显示出更长的总记录时间，并且具有更长的发作持续时间。Fisher大鼠的运动缺陷也增加，与上述结果相关。尽管有这些结果，费舍尔大鼠在受伤后的认知测试中表现更好。结果表明，不同的大鼠品系对损伤的反应不同，因此在临床前神经创伤研究中，应变影响是评估结果时的重要考虑因素。
The present study directly compares the effects of experimental brain injury in two commonly used rat strains: Fisher 344 and Sprague-Dawley. We previously found that Fisher rats have a higher mortality rate and more frequent seizure attacks at the same injury level than Sprague-Dawley rats. Although strain differences in rats are commonly accepted as contributing to variability among studies, there is a paucity of literature addressing strain influence in experimental neurotrauma. Therefore this study compares outcome measures in two rat strains following lateral fluid percussion injury. Fisher 344 and Sprague-Dawley rats were monitored for changes in physiological measurements, intracranial pressure, and electroencephalographic activity. We further analyzed neuronal degeneration and cell death in the injured brain using Fluoro-Jade-B (FJB) histochemistry and caspase-3 immunostaining. Behavioral studies using the beam walk and Morris water maze were conducted to characterize strain differences in both motor and cognitive functional recovery following injury. We found that Fisher rats had significantly higher intracranial pressure, prolonged seizure activity, increased FJB-positive staining in the injured cortex and thalamus, and increased caspase-3 expression than Sprague-Dawley rats. On average, Fisher rats displayed a greater amount of total recording time in seizure activity and had longer ictal durations. The Fisher rats also had increased motor deficits, correlating with the above results. In spite of these results, Fisher rats performed better on cognitive tests following injury. The results demonstrate that different rat strains respond to injury differently, and thus in preclinical neurotrauma studies strain influence is an important consideration when evaluating outcomes.