Succinic acid production by Actinobacillus succinogenes NJ113 using corn steep liquor powder as nitrogen source
Succinic acid production by Actinobacillus succinogenes NJ113 using corn steep liquor powder as nitrogen source
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玉米浆粉为氮源产琥珀酸放线杆菌NJ113生产琥珀酸
DOI：
10.1016/j.biortech.2013.03.107
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发表时间：
2013-05-01
影响因子：
11.4
通讯作者：
Ouyang, Ping-Kai
中科院分区：
文献类型：
--
作者：
Xi, Yong-lan;Chen, Ke-quan;Ouyang, Ping-Kai
以玉米浆粉为氮源，代替传统的琥珀酸生产中使用的酵母抽提物，对产琥珀酸放线杆菌NJ 113进行发酵。此外，当向发酵培养基中加入血红素并以低速搅拌培养物时，从50 g/l的葡萄糖浓度获得37.9 g/l的最大琥珀酸浓度，并实现0.75 g/l/h的生产率。这些产率几乎与用葡萄糖和酵母提取物发酵一样高。这些结果表明，血红素补充CSL可能是一个合适的替代氮源的成本效益的方法生产琥珀酸与A。产琥珀酸酯酶NJ 113，同时比以前的方法消耗更少的能量。(C)2013爱思唯尔有限公司保留所有权利。
In this study, corn steep liquor powder (CSL) was used as nitrogen source to replace the relatively costly yeast extract typically used for the production of succinic acid with Actinobacillus succinogenes NJ113. Moreover, when heme was added to the fermentation medium and the culture was agitated at a low speed, a maximum succinic acid concentration of 37.9 g/l was obtained from a glucose concentration of 50 g/l, and a productivity of 0.75 g/l/h was achieved. These yields are almost as high as for fermentation with glucose and yeast extract. These results suggest that heme-supplemented CSL may be a suitable alternative nitrogen source for a cost-effective method of producing succinic acid with A. succinogenes NJ113 while consuming less energy than previous methods. (C) 2013 Elsevier Ltd. All rights reserved.