A rickettsiales-like infection in the Pacific razor clam, Siliqua patula.
A rickettsiales-like infection in the Pacific razor clam, Siliqua patula.
复制标题
太平洋蛏子（Siliqua patula）中的立克次体样感染。
DOI：
10.1016/0022-2011(84)90048-x
复制
发表时间：
1984
影响因子：
3.4
通讯作者：
M. Peacock
中科院分区：
文献类型：
--
作者：
R. Elston;M. Peacock
在来自华盛顿州太平洋海岸的太平洋蛏子（Siliqua patula）的消化吸收上皮中发现了一种细胞内原核立克次体样生物。在检查的 80 只动物中，30% 含有微生物，所有阳性动物的壳长在 1.5 毫米到 3.0 厘米之间。该生物体呈嗜碱性包涵体，平均直径为 5.0 μm。从超微结构上看，内含物是膜结合的液泡，含有原核形式，平均长度为 0.8 μm，最长可达 1.8 μm。这些生物体具有双层三层膜，似乎形成气泡和外膜囊泡。通过组织学和电子显微镜检查，该生物体是多形性的。宿主对感染的反应似乎仅包括受感染细胞的变圆和分离。对感染蛤消化腺的印迹涂片进行立克次体、普瓦泽基立克次体、伯内蒂立克次体、鹦鹉热衣原体和嗜肺军团菌的特异性免疫荧光检测，结果均为阴性。
An intracellular procaryotic rickettsiales-like organism was discovered in the digestive absorptive epithelium of the Pacific razor clam,Siliqua patula, from the Pacific Coast of Washington State. Of 80 animals examined, 30% contained the organisms, and all positive animals were between 1.5 mm and 3.0 cm shell length. The organisms appeared as basophilic inclusions averaging 5.0 μm in diameter. Ultrastructurally, the inclusions were membrane-bound vacuoles containing procaryotic forms which averaged 0.8 μm by up to 1.8 μm in length. These organisms had a double layer of trilaminar membrane which appeared to form blebs and outer membrane vesicles. The organisms were pleomorphic by both histological and electron microscopical examination. The host response to infection appeared to consist only of the rounding and detachment of the infected cells. Specific immunofluorescent tests forRickettsia rickettsia, R. prowazekii, Coxiella burnetti, Chlamydia psittaci, andLegionella pneumophilawere all negative when tested on impression smears of infected clam digestive gland.