Comparative studies on the biology of Actinobacillus actinomycetemcomitans leukotoxin in primates.
Comparative studies on the biology of Actinobacillus actinomycetemcomitans leukotoxin in primates.
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灵长类动物伴放线放线菌白毒素生物学的比较研究。
DOI：
10.1111/j.1399-302x.1987.tb00270.x
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发表时间：
1987
影响因子：
--
通讯作者：
Slots,J
中科院分区：
文献类型：
--
作者：
Taichman,NS;Simpson,DL;Sakurada,S;Cranfield,M;DiRienzo,J;Slots,J
这些研究涉及不同灵长类动物放线共生放线杆菌毒素的生物学特性。我们比较了灵长类动物多形核白细胞（PMN）对白细胞毒素的敏感性，并确定动物血清中是否有毒素中和抗体。此外，对代表性非人灵长类动物的龈下菌斑进行了白细胞毒素A分析。不同灵长类动物的中性粒细胞对放线菌的白细胞毒性提取物的敏感性存在显著差异。伴放线菌人类、类人猿和大多数旧大陆猴子的中性粒细胞在体外暴露于毒素时被破坏。相比之下，原猴族、新世界猴和小型猿类的中性粒细胞对白细胞毒素不敏感。与非人灵长类动物的PMN相比，人PMN更敏感。就对毒素的敏感性而言，黑猩猩的PMNs最接近人类的PMNs。这些数据表明，白细胞毒素对灵长类动物中性粒细胞具有独特的特异性，对人类白细胞尤其有效。绝大多数成年灵长类动物的血清都能中和A.放线菌伴放线菌毒素，包括其中性粒细胞对该毒素具有抗性的动物。这表明大多数非人灵长类动物都暴露于产毒素的A型菌株。放线菌共生体，这是证实了代表性的物种，其中leukotoxicA。从龈下菌斑样品中可分离出放线菌伴菌。相比之下，不到20%的正常人血清中和了白细胞毒素，而超过90%的青少年牙周炎患者血清具有相对较高的毒素中和抗体升数。似乎大多数成年灵长类动物都携带A。放线菌共生菌并产生针对该生物体的白细胞毒素的抗体。另一方面，在人类中，这些现象通常与破坏性牙周病有关。人类和非人类灵长类动物在对白细胞毒素的敏感性和/或免疫反应动力学方面的差异可能是解释为什么这种生物体似乎属于某些灵长类动物正常的本土口腔微生物群落，但在人类中作为牙周病原体的重要决定因素。
These studies concern the biological properties of theActinobacillus actinomycetemcomitansleukotoxin in different primates. We compared the sensitivity of primate polymorphonuclear leukocytes (PMNs) to the leukotoxin and determined whether animals had toxin‐neutralizing antibodies in serum. In addition, subgingival plaques from representative non‐human primates were analysed for leukotoxin‐producingA. actinomycetemcomitans.PMNs from different primates varied significantly in sensitivity to leukotoxic extracts of A. actinomycetemcomitans. Neutrophils from humans, the Great Apes and most Old World Monkeys were destroyed uponin vitroexposure to the toxin. By contrast, PMNs from Prosimii, New World Monkeys and the Lesser Apes were not sensitive to the leukotoxin. Human PMNs were more sensitive when compared to PMNs of non‐human primates. Chimpanzee PMNs were closest to human PMNs in terms of sensitivity to the toxin. The data indicate that the leukotoxin has unique specificites for primate PMNs and is especially potent against human leukocytes. Sera from the vast majority of adult primates were capable of neutralizing theA. actinomycetemcomitansleukotoxin, including animals whose PMNs were resistant to the toxin. This suggests that most non‐human primates have been exposed to toxin‐producing strains ofA. actinomycetemcomitans, and this was confirmed in representative species where leukotoxicA. actinomycetemcomitanscould be isolated from subgingival plaque samples. By contrast, less than 20% of sera from normal humans neutralized the leukotoxin whereas over 90% of sera from juvenile periodontitis patients had relatively high toxin‐neutralizing antibody liters. It appears that the majority of adult primates harbor A. actinomycetemcomitans and produce antibodies against the leukotoxin of this organism. In humans, on the other hand, these phenomena are usually associated with destructive periodontal disease. Differences between man and non‐human primates in terms of their sensitivity to and/ or in the dynamics of the immune response against the leukotoxin, may be important determinants in explaining why this organism seems to belong to the normal indigenous oral microflora in some primates but acts as a periodontal pathogen in man.