GENETIC UNIFORMITY IN 2 POPULATIONS OF DROSOPHILA-MELANOGASTER AS REVEALED BY FILTER HYBRIDIZATION OF 4-NUCLEOTIDE-RECOGNIZING RESTRICTION ENZYME DIGESTS
GENETIC UNIFORMITY IN 2 POPULATIONS OF DROSOPHILA-MELANOGASTER AS REVEALED BY FILTER HYBRIDIZATION OF 4-NUCLEOTIDE-RECOGNIZING RESTRICTION ENZYME DIGESTS
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DOI：
10.1073/pnas.83.10.3562
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
AGUADE, M
中科院分区：
文献类型：
--
作者：
KREITMAN, M;AGUADE, M
描述了一种过滤杂交方法，通过使用识别四核苷酸序列的限制酶和用于分离片段的变性聚丙烯酰胺凝胶来鉴定限制位点和插入/缺失变异。87线黑腹果蝇代表两个自然种群进行了调查，在2.7-脱氢酶区域，包括乙醇脱氢酶位点。从17个限制性位点和11个插入/缺失多态性和一个等位酶多态性中确定了50种不同的单倍型。没有证据表明东海岸和西海岸（北美）样本之间存在遗传分化。该技术在DNA多态性筛选中有广泛的应用。
A filter hybridization method is described for identifying restriction-site and insertion/deletion variation by using restriction enzymes that recognize four-nucleotide sequences and denaturing polyacrylamide gels for separating fragments. Eighty-seven lines of Drosophila melanogaster representing two natural populations were surveyed over a 2.7-kilobase region encompassing the alcohol dehydrogenase locus. Fifty distinct haplotypes were identified from 17 restriction-site and 11 insertion/deletion polymorphisms and from one allozyme polymorphism. There was no evidence for genetic differentiation between an East-Coast and a West-Coast (North American) sample. This technique has widespread applications in screening for DNA polymorphism.