Ontogenesis of growth hormone-releasing hormone neurons in the rat hypothalamus.
Ontogenesis of growth hormone-releasing hormone neurons in the rat hypothalamus.
复制标题
大鼠下丘脑生长激素释放激素神经元的个体发生。
DOI：
10.1159/000124579
复制
发表时间：
1986
影响因子：
4.1
通讯作者：
Frohman,LA
中科院分区：
文献类型：
--
作者：
Ishikawa,K;Katakami,H;Jansson,JO;Frohman,LA
使用特异性抗大鼠生长激素释放激素（GH-RH）血清，通过免疫组织化学方法研究了大鼠下丘脑中含生长激素释放激素（GH-RH）神经元的个体发生。免疫反应性纤维首次检测到的前瞻性正中隆起在妊娠第18天。在随后的3天内，它们在该结构内的分布和染色强度迅速增加。在第21天，阳性纤维也可见于弓状核内的神经丛。在1日龄的大鼠，秋水仙碱处理，阳性细胞体分布在几个下丘脑核团，包括弓状核，背内侧核，下丘脑基底外侧，穹窿周围区。分布与先前在成年大鼠中描述的相似。在不同的下丘脑区域染色的强度增加，在出生后的早期生活的水平几乎相当于成年大鼠的30天。这些结果表明，早期出现的GH-RH阳性终端的正中隆起和广泛分布的胞体在出生后的生命的早期阶段支持的观点，下丘脑GH-RH担任一个重要的作用，在调节生长激素的分泌在产前晚期和新生儿早期。
The ontogenesis of growth hormone releasing hormone (GH-RH) containing neurons in the rat hypothalamus has been studied by immunohistochemistry, using a specific anti-rat GH-RH serum. Immunoreactive fibers were first detected in the prospective median eminence on day 18 of gestation. During the subsequent 3 days, they rapidly increased in distribution and intensity of staining within this structure. On day 21, positive fibers were also visible in a plexus within the arcuate nucleus. In 1-day-old rats treated with colchicine, positive perikarya were distributed in several hypothalamic nuclei, including the arcuate nucleus, dorsomedial nucleus, basal lateral hypothalamus, and perifornical region. The distribution was similar to that previously described in adult rats. The intensity of staining in the various hypothalamic regions increased during early postnatal life to levels nearly comparable to those in adult rats by 30 days. These findings showing the early appearance of GH-RH-positive terminals in the median eminence and the wide distribution of the perikarya at an early stage of postnatal life support the view that hypothalamic GH-RH serves an important role in the regulation of growth hormone secretion during late prenatal and early neonatal periods.