Further characterization of human salivary anticandidal activities in a human immunodeficiency virus-positive cohort by use of microassays.
Further characterization of human salivary anticandidal activities in a human immunodeficiency virus-positive cohort by use of microassays.
复制标题
通过使用微量测定进一步表征人类免疫缺陷病毒阳性群体中人类唾液抗念珠菌活性。
DOI：
10.1128/cdli.6.6.851-855.1999
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Yeh,CK
中科院分区：
文献类型：
--
作者：
Lin,AL;Shi,Q;Johnson,DA;Patterson,TF;Rinaldi,MG;Yeh,CK
Salivary anticandidal activities play an important role in oral candidal infection. R. P. Santarpia et al. (Oral Microbiol. Immunol. 7:38–43, 1992) developed in vitro anticandidal assays to measure the ability of saliva to inhibit the viability ofCandida albicansblastoconidia and the formation of germ tubes byC. albicans. In this report, we describe modifications of these assays for use with small volumes of saliva (50 to 100 μl). For healthy subjects, there is strong inhibition of blastoconidial viability in stimulated parotid (75%), submandibular-sublingual (74%), and whole (97%) saliva, as well as strong inhibition of germ tube formation (>80%) for all three saliva types. The susceptibility of severalCandidaisolates to inhibition of viability by saliva collected from healthy subjects is independent of body source ofCandidaisolation (blood, oral cavity, or vagina) or the susceptibility of the isolate to the antifungal drug fluconazole. Salivary anticandidal activities in human immunodeficiency virus (HIV)-infected patients were significantly lower than those in healthy controls for inhibition of blastoconidial viability (P< 0.05) and germ tube formation (P< 0.001). Stimulated whole-saliva flow rates were also significantly lower (P< 0.05) for HIV-infected patients. These results show that saliva of healthy individuals has anticandidal activity and that this activity is reduced in the saliva of HIV-infected patients. These findings may help explain the greater incidence of oral candidal infections for individuals with AIDS.
