In vitro pattern formation during neurogenesis in neuroectodermal progenitor cells immortalized by p53-deficiency
In vitro pattern formation during neurogenesis in neuroectodermal progenitor cells immortalized by p53-deficiency
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DOI：
10.1016/s0736-5748(97)00015-4
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发表时间：
1997-10-01
影响因子：
1.8
通讯作者：
Madarasz, E
中科院分区：
文献类型：
--
作者：
Schlett, K;Herberth, B;Madarasz, E
在体外神经分化诱导p53缺陷的永生化神经外胚层祖细胞系，NE-4C，通过处理与视黄酸[K. Schlett和E. Madarasz（1997）J. Neurosci. Res. 47，405-416]。巢蛋白丝的重排是神经元形成的早期标志。神经丝蛋白含量的增加伴随着诱导前体中巢蛋白丝表达的减少。与神经元的出现相比，具有星形胶质细胞特征的细胞的出现延迟4-5天。未来神经元的分选类似于体内神经前体的分离。细胞的形状和位置的连续变化导致形成特征性的形态学图案。根据形态学变化，将体外神经分化分为5个阶段，形态学变化分析表明，细胞间相互作用在诱导前体细胞的命运决定中起着重要作用。我们的观察结果表明，NE-4C细胞系可以作为一个体外模型，以研究神经发生的一些早期步骤。(C)1997年ISDN。
In vitro neural differentiation was induced in a p53-deficient immortalized neuroectodermal progenitor cell line, NE-4C, by treatment with retinoic acid [K. Schlett and E. Madarasz (1997) J. Neurosci. Res. 47, 405-416]. Rearrangement of nestin filaments was an early marker of neuron-formation. The increase in neurofilament protein content was accompanied by a decrease in the expression of nestin filaments in induced precursors. Cells with astroglial features appeared with a delay of 4-5 days compared to the appearence of neurons.Future neurons were sorted out from the substrate-attached population of apparently non-induced cells. The sorting out of future neurons resembled the separation of neural precursors in vivo. The continuous changes in the shape and also in the position of the cells resulted in the formation of characteristic morphological patterns. On the basis of morphological changes, five characteristic stages of in vitro neural differentiation were distinguished.The analysis of the morphological changes revealed that cell-to-cell interactions played an essential role in the cell fare decision made by induced precursors. Our observations indicate that the NE-4C cell line can serve as an in vitro model to investigate some early steps of neurogenesis. (C) 1997 ISDN.