Response of circulating tumor cells to systemic therapy in patients with metastatic breast cancer:: Comparison of quantitative polymerase chain reaction and immunocytochemical techniques
Response of circulating tumor cells to systemic therapy in patients with metastatic breast cancer:: Comparison of quantitative polymerase chain reaction and immunocytochemical techniques
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DOI：
10.1200/jco.2000.18.7.1432
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发表时间：
2000-04-01
影响因子：
45.3
通讯作者：
Coombes, RC
中科院分区：
文献类型：
--
作者：
Smith, BM;Slade, MJ;Coombes, RC
目的：我们以前开发了一个定量系统，用于检测细胞角蛋白19（CK-19）的成绩单，使用逆转录聚合酶链反应（PCR）检测血液和骨髓中的乳腺癌细胞。本研究的目的是确定该系统在监测转移性疾病患者中的价值，并将其与已建立的免疫细胞化学方法进行比较。患者和方法：招募了进行性、局部晚期和转移性乳腺癌（均为IV期）患者，这些患者应开始全身治疗。在整个疗程中，采用定量PCR（QPCR）和免疫细胞化学（ICC）对血液样本进行CK-19转录物分析。133份标本中，QPCR阳性72份（49.6%），ICC阳性56份（42%）。在采用两种技术分析的133份标本中，95份（71.4%）的结果相同，在阳性的II样本中，40份（56%）采用两种方法均为阳性。检测到的细胞数量与QPCR值之间的关系具有统计学显著性（P < .0001）。在15个可评估的治疗过程中，15个治疗结局（缓解或疾病进展）中的17个（68%）通过QPCR测量反映，21个治疗结局中的12个（57%）通过ICC反映。在研究过程中，5例患者显示ct缓解，其中4例（80%）IGC一致，3例（60%）QPCR一致。18个疗程的治疗导致疾病的进展，但是，只有15个这些是可评估的ICC，ICC是在协议中的8个（53%）的15;这些情况下，和QPCR在15（83%）的18 cases.Conclusion：循环癌细胞经常发现在转移性乳腺癌患者。在大多数患者中，通过QPCR或ICC评估的癌细胞数量反映了全身治疗的结果。(C)2000年，美国临床肿瘤学会。
Purpose: We previously developed a quantitative system for the detection of cytokeratin 19 (CK-19) transcripts using reverse transcriptase polymerase chain reaction (PCR) to detect breast carcinoma cells in blood and bone marrow. The aim of this study war to determine the value of this system in monitoring patients with metastatic disease and to compare it with an established immunocytochemical method,Patients and Methods: Patients with progressive, locally advanced, and metastatic breast cancer (all stage IV) who were due to start systemic treatment were recruited. Blood samples were analyzed for CK-19 transcripts using quantitative PCR (QPCR) and immunocytochemistry (ICC) throughout their course of treatment.Results: One hundred forty-five blood samples were obtained from 22 patients over 13 months. Seventy-two (49.6%) of there samples were positive by QPCR, and 56 (42%) of 133 were positive by ICC. Of the 133 specimens analyzed by both techniques, 95 (71.4%) had the same results for each, and of the II samples that were positive, 40 (56%) were positive by both methods. The relationship between the number of cells detected and the QPCR values was statistically significant (P < .0001). Of the 15 courses of assessable treatment, 17 (68%) of 15 treatment outcomes (either response or disease progression) were reflected by QPCR measurements, and 12 (57%) of 21 were reflected by ICC, During the course of the study, five patients showed ct response, and of these, IGC was in agreement in four cases (80%) and QPCR in three cares (60%). Eighteen courses of treatment resulted in progression of the disease; however, only 15 of these were assessable by ICC, ICC was in agreement in eight (53%) of 15 of; these cases, and QPCR in 15 (83%) of 18 cases.Conclusion: Circulating carcinoma cells are frequently found in patients with metastatic breast cancer. In the majority of patients, cancer cell numbers or evaluated by QPCR or ICC reflected the outcome of systemic treatment. (C) 2000 by American Society of Clinical Oncology.