Tight control of mitochondrial membrane potential by cytochrome c oxidase
Tight control of mitochondrial membrane potential by cytochrome c oxidase
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DOI：
10.1016/j.mito.2010.12.004
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发表时间：
2011-03-01
期刊：
影响因子：
4.4
通讯作者：
Villani, Gaetano
中科院分区：
文献类型：
--
作者：
Pacelli, Consiglia;Latorre, Dominga;Villani, Gaetano
在本工作中，我们严格地考察了KCN滴定技术在研究细胞色素c氧化酶(COX)在线粒体膜电位(Delta Psi(Mito))存在下对细胞呼吸的控制方面的应用。我们清楚地表明，在存在最大Delta psi(Mito)的情况下，COX的明显抑制阈值是由于KCN诱导的Delta psi(Mito)的降低，而不是由于COX对线粒体呼吸的低控制。COX对Delta psi(Mito)的严密控制为理解人类神经肌肉退行性疾病中线粒体缺陷的发病机制提供了进一步的见解。(C)2010年Elsevier B.V.和线粒体研究会。版权所有。
In the present work we have critically examined the use of the KCN-titration technique in the study of the control of the cellular respiration by cytochrome c oxidase (COX) in the presence of the mitochondrial membrane potential (Delta Psi(mito)) in HepG2 cells. We clearly show that the apparent high inhibition threshold of COX in the presence of maximal Delta psi(mito), is due to the KCN-induced decrease of Delta psi(mito), and not to a low control of COX on the mitochondrial respiration. The tight control exerted by COX on the Delta psi(mito) provides further insights for understanding the pathogenetic mechanisms associated with mitochondrial defects in human neuromuscular degenerative disorders. (C) 2010 Elsevier B.V. and Mitochondria Research Society. All rights reserved.