SERK and APOSTART.: Candidate genes for apomixis in Poa pratensis
SERK and APOSTART.: Candidate genes for apomixis in Poa pratensis
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DOI：
10.1104/pp.105.062059
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发表时间：
2005-08-01
期刊：
影响因子：
7.4
通讯作者：
Falcinelli, M
中科院分区：
文献类型：
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作者：
Albertini, E;Marconi, G;Falcinelli, M
种子生产通常需要异性配子的交配。无融合生殖是一种无性生殖方式，避免了减数分裂和卵受精。无融合生殖的基本特征是胚胎是由无融合生殖产生的胚囊的未减数卵通过孤雌生殖自主形成的。如果对无融合生殖有很好的理解和利用，它就可以被用来无限繁殖上级杂种或携带复杂基因组的特定基因型。更深入地了解调节生殖事件的机制将从根本上有助于理解无融合生殖途径的遗传控制。在草地早熟禾中，我们分离并鉴定了两个基因，PpSERK（体细胞胚胎发生受体样激酶）和APOSTART。通过快速扩增cDNA末端恢复这些全长基因，并通过逆转录-聚合酶链反应和原位杂交分别评估其时空表达模式。PpSERK和APOSTART的表达在无融合生殖和有性生殖基因型中存在差异。它们在植物孢子发生、配子发生和胚胎发生过程中所需的细胞信号转导级联和贩运事件中的假定作用被报道和讨论。我们建议，在珠心细胞的无融合生殖基因型，PpSERK是开关，渠道胚囊的发展，它也可以重定向信号基因产物的车厢以外的典型的。APOSTART参与减数分裂和程序性细胞死亡也进行了讨论。
Seed production generally requires the mating of opposite sex gametes. Apomixis, an asexual mode of reproduction, avoids both meiotic reduction and egg fertilization. The essential feature of apomixis is that an embryo is formed autonomously by parthenogenesis from an unreduced egg of an embryo sac generated through apomeiosis. If apomixis were well understood and harnessed, it could be exploited to indefinitely propagate superior hybrids or specific genotypes bearing complex gene sets. A more profound knowledge of the mechanisms that regulate reproductive events would contribute fundamentally to understanding the genetic control of the apomictic pathway. In Poa pratensis, we isolated and characterized two genes, PpSERK (SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE) and APOSTART. These full-length genes were recovered by rapid amplification of cDNA ends and their temporal and spatial expression patterns were assessed by reverse transcription-polymerase chain reaction and in situ hybridization, respectively. The expression of PpSERK and APOSTART differed in apomictic and sexual genotypes. Their putative role in cell-signaling transduction cascades and trafficking events required during sporogenesis, gametogenesis, and embryogenesis in plants is reported and discussed. We propose that, in nucellar cells of apomictic genotypes, PpSERK is the switch that channels embryo sac development and that it may also redirect signaling gene products to compartments other than their typical ones. The involvement of APOSTART in meiosis and programmed cell death is also discussed.