The ABCF gene family facilitates disaggregation during animal development
The ABCF gene family facilitates disaggregation during animal development
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DOI：
10.1091/mbc.e19-08-0443
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发表时间：
2020-04
影响因子：
3.3
通讯作者：
Sydney Skuodas;A. Clemons;Michael H. Hayes;A. Goll;B. Zora;D. L. Weeks;B. Phillips;J. Fassler
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文献类型：
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作者：
Sydney Skuodas;A. Clemons;Michael H. Hayes;A. Goll;B. Zora;D. L. Weeks;B. Phillips;J. Fassler
蛋白质聚集，曾经被认为是一个预兆和/或压力，年龄和病理条件的后果，正在出现作为一个新的概念，在细胞调节。正常与病理性聚集可以通过细胞调节聚集体的形成、修饰和溶解的能力来区分。我们发现秀丽隐杆线虫聚集体在大细胞/卵裂球（卵母细胞，胚胎）和较小的，进一步分化的细胞（原始生殖细胞）中观察到，并且使用细胞生物学和遗传学工具对其进行分析是简单的。这些观察结果与聚集体参与正常发育的假设一致。在酿酒酵母中使用跨平台分析，C。elegans和非洲爪蟾，我们目前的研究确定了一个新的解聚酶家族编码的动物基因组和胚胎表达。我们的初步分析酵母Arb 1/Abcf 2在解聚和动物ABCF蛋白在胚胎发生的可能性是一致的，ABCF基因家族的成员可能编码解聚所需的聚合物加工在动物发育的最早阶段。
Protein aggregation, once believed to be a harbinger and/or consequence of stress, age, and pathological conditions, is emerging as a novel concept in cellular regulation. Normal versus pathological aggregation may be distinguished by the capacity of cells to regulate the formation, modification, and dissolution of aggregates. We find that Caenorhabditis elegans aggregates are observed in large cells/blastomeres (oocytes, embryos) and in smaller, further differentiated cells (primordial germ cells), and their analysis using cell biological and genetic tools is straightforward. These observations are consistent with the hypothesis that aggregates are involved in normal development. Using cross-platform analysis in Saccharomyces cerevisiae, C. elegans, and Xenopus laevis, we present studies identifying a novel disaggregase family encoded by animal genomes and expressed embryonically. Our initial analysis of yeast Arb1/Abcf2 in disaggregation and animal ABCF proteins in embryogenesis is consistent with the possibility that members of the ABCF gene family may encode disaggregases needed for aggregate processing during the earliest stages of animal development.