Tea polyphenol epigallocatechin gallate inhibits Escherichia coli by increasing endogenous oxidative stress
Tea polyphenol epigallocatechin gallate inhibits Escherichia coli by increasing endogenous oxidative stress
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茶多酚表没食子儿茶素没食子酸酯通过增加内源性氧化应激抑制大肠杆菌
DOI：
10.1016/j.foodchem.2016.08.098
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发表时间：
2017-02-15
期刊：
影响因子：
8.8
通讯作者：
Liu, Zhong-Hua
中科院分区：
文献类型：
--
作者：
Xiong, Li-Gui;Chen, Yi-Jun;Liu, Zhong-Hua
茶多酚表没食子儿茶素没食子酸酯（EGCG）是一种具有许多潜在健康益处的常见植物化学物质，其抗菌作用是众所周知的。然而，其杀菌作用的机制仍不清楚。使用大肠大肠杆菌作为模式生物，在这里认为，H2O2的合成由表没食子儿茶素没有归因于其抑制作用。与此相反，EGCG的杀菌作用是由于细胞内活性氧的增加和适应性氧化应激反应的减弱。大肠杆菌中，由于抗氧化剂N-乙酰半胱氨酸的共同管理，而不是由于外源过氧化氢酶。此外，我们注意到EGCG与百草枯联合使用时具有协同杀菌作用。然而，在厌氧条件下，EGCG的抑制作用被阻止。总之，EGCG引起E.大肠杆菌，从而抑制其生长，因此使用表没食子儿茶素没食子酸酯作为促氧化剂是支持这项研究。(C)2016爱思唯尔有限公司版权所有。
The antibacterial effects of tea polyphenol epigallocatechin gallate (EGCG), a common phytochemical with a number of potential health benefits, are well known. However, the mechanism of its bactericidal action remains unclear. Using E. coli as a model organism, it is argued here that H2O2 synthesis by EGCG is not attributed to its inhibitory effects. In contrast, the bactericidal action of EGCG was a result of increased intracellular reactive oxygen species and blunted adaptive oxidative stress response in E. coli due to the co-administration of antioxidant N-acetylcysteine, and not on account of exogenous catalase. Furthermore, we noted a synergistic bactericidal effect for EGCG when combined with paraquat. However, under anaerobic conditions, the inhibitory effect of EGCG was prevented. In conclusion, EGCG caused an increase in endogenous oxidative stress in E. coli, thereby inhibiting its growth, and hence the use of EGCG as a prooxidant is supported by this study. (C) 2016 Elsevier Ltd. All rights reserved.