Structural characterisation of N-linked glycan mixtures by precursor ion scanning and tandem mass spectrometric analysis
Structural characterisation of N-linked glycan mixtures by precursor ion scanning and tandem mass spectrometric analysis
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DOI：
10.1002/(sici)1097-0231(19990730)13:14
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发表时间：
1999-01-01
影响因子：
2
通讯作者：
Camilleri, P
中科院分区：
文献类型：
--
作者：
Charlwood, J;Langridge, J;Camilleri, P
通过混合四极杆正交加速飞行时间质谱仪 (Q-Tof) 在前体离子扫描和串联质谱 (MS/MS) 模式下直接分析 2-氨基吖啶酮 (2-AMAC) 标记的 N-连接聚糖库。在该仪器上使用前体离子扫描策略提供了一种快速、灵敏的筛选聚糖混合物的方法，无需事先通过色谱方法进行分离，它可以轻松、初步地表征聚糖将聚糖分为不同类别，例如高甘露糖或复合聚糖，在初始前体离子扫描模式下获得每种聚糖的初步测序信息，但可以使用 MS/MS 模式获得所选聚糖的进一步测序信息。版权所有 (C) 1999 John Whey & Sons, Ltd.
2-Aminoacridone (2-AMAC) labelled N-linked glycan pools were analysed directly by a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer (Q-Tof) in the precursor ion scanning and tandem mass spectrometry (MS/MS) modes, The use of a precursor ion scanning strategy on this instrument provides a rapid and sensitive method of screening glycan mixtures, without prior separation by chromatographic methods, It allows facile and preliminary characterisation of glycans into different classes, for example, high-mannose or complex glycans, Preliminary sequencing information for each glycan is obtained in the initial precursor ion scanning mode, but further sequencing information of selected glycans can be obtained using the MS/MS mode. Copyright (C) 1999 John Whey & Sons, Ltd.