Functional Mechanisms of ABC Transporters as Revealed by Molecular Simulations
Functional Mechanisms of ABC Transporters as Revealed by Molecular Simulations
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DOI：
10.1007/978-981-10-8459-1_12
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
T. Furuta;M. Sakurai
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文献类型：
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作者：
T. Furuta;M. Sakurai
细胞中的主动运输是由一类被称为atp结合盒（ABC）转运蛋白的整体膜蛋白完成的。驱动这些分子机器的能量来源是ATP分子与核苷酸结合域（nbd）结合产生的自由能，以及ATP水解产生的自由能。nbd的打开和关闭运动是由这些能量驱动的，这些能量通过机械传动段（耦合螺旋）通过跨膜结构域（TMDs）传播。因此，产生了tmd的打开和关闭运动，从而允许底物的摄取和释放。在这些过程中，ATP的化学能转化为机械运动，是一个典型的化学-机械耦合的例子。在这篇综述中，我们描述了目前对这种耦合机制的理解，重点介绍了ATP和水的合作作用。
Active transport in cells is accomplished by a class of integral membrane proteins known as ATP-binding cassette (ABC) transporters. The energy source powering these molecular machines is the free energy generated by the binding of ATP molecules to nucleotide-binding domains (NBDs), as well as the free energy generated by ATP hydrolysis. The opening and closing motions of the NBDs are driven by these energies, which are propagated through transmembrane domains (TMDs) via mechanical transmission segments (coupling helices). As a result, the opening and closing motions of the TMDs are generated, which allow the uptake and release of substrates. In these processes, the chemical energy of ATP is converted into mechanical motion, a typical example of chemo-mechanical coupling. In this review, we describe the current understanding of this coupling mechanism, with a focus on the cooperative role of ATP and water.