Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.
Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.
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DOI：
10.1096/fj.202100122r
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Darszon A
中科院分区：
文献类型：
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作者：
Sánchez-Cárdenas C;Romarowski A;Orta G;De la Vega-Beltrán JL;Martín-Hidalgo D;Hernández-Cruz A;Visconti PE;Darszon A
我们最近报道了两种不同的方法，提高精子的功能。第一种方法涉及短暂暴露于Ca2+离子载体A23187，第二种方法需要在没有能量营养物（饥饿）的情况下孵育精子。这两种方法都与运动性的最初损失相关，然后分别进行涉及离子载体去除或添加能量代谢物的救援步骤。在目前的工作中，我们表明，饥饿是伴随着细胞内Ca 2+（[Ca 2 +]i）的增加。此外，饥饿的细胞获得了显著增强的能力，经历胆甾酮诱导的顶体反应。电生理测量表明，CatSper在饥饿条件下仍然活跃。然而，在CatSper敲除小鼠的精子中也观察到[Ca2+]i增加。饥饿时，除了能量营养逆转[Ca 2 +]i的影响，并降低孕酮对顶体反应的影响，以控制水平。这些数据表明，这两种方法具有共同的分子特征。
We have recently reported two different methodologies that improve sperm functionality. The first method involved transient exposure to the Ca2+ ionophore A23187, the second required sperm incubation in the absence of energy nutrients (starvation). Both methods were associated with an initial loss of motility followed by a rescue step involving ionophore removal or addition of energy metabolites, respectively. In the present work, we show that starvation is accompanied by an increase in intracellular Ca2+ ([Ca2+]i). Additionally, the starved cells acquire a significantly enhanced capacity to undergo a progesterone-induced acrosome reaction. Electrophysiological measurements show that CatSper remains active in starvation conditions. However, the increase in [Ca2+]i was also observed in sperm from CatSper null mice. Upon starvation, addition of energy nutrients reversed the effects on [Ca2+]i and decreased the effect of progesterone on the acrosome reaction to control levels. These data indicate that both methods have common molecular features.