CLONING OF HUMAN TUMOR-NECROSIS-FACTOR (TNF) RECEPTOR CDNA AND EXPRESSION OF RECOMBINANT SOLUBLE TNF-BINDING PROTEIN
CLONING OF HUMAN TUMOR-NECROSIS-FACTOR (TNF) RECEPTOR CDNA AND EXPRESSION OF RECOMBINANT SOLUBLE TNF-BINDING PROTEIN
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DOI：
10.1073/pnas.87.19.7380
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发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
FELDMANN, M
中科院分区：
文献类型：
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作者：
GRAY, PW;BARRETT, K;FELDMANN, M
已分离到人肿瘤坏死因子（TNF）受体之一的cDNA。该cDNA编码一种由455个氨基酸组成的蛋白质，该蛋白质被分为由171个残基组成的胞外结构域和由221个残基组成的胞质结构域。细胞外结构域已被设计用于在哺乳动物细胞中表达，该重组衍生物结合tnf . α。具有高亲和力，并能抑制其体外细胞毒活性。TNF受体与细胞表面蛋白家族具有相似性，包括神经生长因子受体、人b细胞表面抗原CD40和大鼠t细胞表面抗原OX40。TNF受体在细胞外部分含有四个富含半胱氨酸的亚结构域。转染了整个TNF受体cDNA的哺乳动物细胞结合放射性标记的TNF. α。亲和力为2.5倍。这种结合可以被未标记的tnf . α竞争性地抑制。或淋巴蛋白（tnf . β）。
The cDNA for one of the receptors for human tumor necrosis factor (TNF) has been isolated. This cDNA encodes a protein of 455 amino acids that is divided into an extracellular domain of 171 residues and a cytoplasmic domain of 221 residues. The extracellular domain has been engineered for expression in mammalian cells, and this recombinant derivative binds TNF.alpha. with high affinity and inhibits its cytotoxic activity in vitro. The TNF receptor exhibits similarity with a family of cell surface proteins that includes the nerve growth factor receptor, the human B-cell surface antigen CD40, and the rat T-cell surface antigen OX40. The TNF receptor contains four cysteine-rich subdomains in the extracellular portion. Mammalian cells transfected with the entire TNF receptor cDNA bind radiolabeled TNF.alpha. with an affinity of 2.5 .times. 10-9 M. This binding can be competitively inhibited with unlabeled TNF.alpha. or lymphotoxin (TNF.beta.).