Silver nanospheres are cytotoxic and genotoxic to fish cells.
Silver nanospheres are cytotoxic and genotoxic to fish cells.
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DOI：
10.1016/j.aquatox.2009.11.016
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发表时间：
2010-04-01
期刊：
影响因子：
--
通讯作者：
Mason MD
中科院分区：
文献类型：
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作者：
Wise JP Sr;Goodale BC;Wise SS;Craig GA;Pongan AF;Walter RB;Thompson WD;Ng AK;Aboueissa AM;Mitani H;Spalding MJ;Mason MD
纳米颗粒由于其独特的物理性质而被广泛研究用于一系列应用。例如，银纳米颗粒因其抗细菌和抗真菌特性而用于商业产品中。其中一些产品可能导致银纳米颗粒进入水生环境。因此，纳米颗粒对人类和水生物种构成健康问题。我们使用青鳉（Oryzias latipes）细胞系研究直径为30 nm的银纳米球的细胞毒性和遗传毒性。在集落形成试验中，0.05、0.3、0.5、3和5 μg/cm 2的处理分别诱导80%、45.7%、24.3%、1%和0.1%的存活。银纳米颗粒也诱导染色体畸变和非整倍体。0、0.05、0.1和0.3 μg/cm 2的处理分别在8%、10.8%、16%和15.8%的中期细胞中诱导损伤，在100个中期细胞中诱导10.8%、15.6%、24%和24%的总畸变。这些数据表明，银纳米颗粒对鱼细胞具有细胞毒性和遗传毒性。
Nanoparticles are being widely investigated for a range of applications due to their unique physical properties. For example, silver nanoparticles are used in commercial products for their antibacterial and antifungal properties. Some of these products are likely to result in silver nanoparticles reaching the aquatic environment. As such, nanoparticles pose a health concern for humans and aquatic species. We used a medaka (Oryzias latipes) cell line to investigate the cytotoxicity and genotoxicity of 30 nm diameter silver nanospheres. Treatments of 0.05, 0.3, 0.5, 3 and 5 μg/cm2 induced 80, 45.7, 24.3, 1 and 0.1% survival, respectively, in a colony forming assay. Silver nanoparticles also induced chromosomal aberrations and aneuploidy. Treatments of 0, 0.05, 0.1 and 0.3 μg/cm2 induced damage in 8, 10.8, 16 and 15.8% of metaphases and 10.8, 15.6, 24 and 24 total aberrations in 100 metaphases, respectively. These data show that silver nanoparticles are cytotoxic and genotoxic to fish cells.