CD24, a glycosylphosphatidylinositol-anchored molecule, is transiently expressed during the development of human central nervous system and is a marker of human neural cell lineage tumors
CD24, a glycosylphosphatidylinositol-anchored molecule, is transiently expressed during the development of human central nervous system and is a marker of human neural cell lineage tumors
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DOI：
10.1007/s004010050442
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发表时间：
1996-04-01
影响因子：
12.7
通讯作者：
FigarellaBranger, D
中科院分区：
文献类型：
--
作者：
Poncet, C;Frances, V;FigarellaBranger, D
CD 24是一种糖蛋白，具有不寻常的结构，由广泛糖基化的小蛋白核心组成，并通过糖基磷脂酰肌醇（GPI）脂质锚与质膜外表面连接。其鼠同源物mCD 24在造血和神经细胞谱系的发育和分化期间瞬时表达。我们已经寻找了CD 24在发育和成熟人脑以及广泛的神经外胚层肿瘤中的表达。神经母细胞瘤，一个亚组的肿瘤能够成熟，从未分化的功能成熟的神经节细胞瘤，更广泛的研究。免疫组织化学研究表明，CD 24是短暂表达的神经元在人脑发育过程中。在神经外胚层肿瘤中，CD 24是神经元肿瘤的标志物。此外，在神经母细胞瘤中，CD 24表达随着肿瘤分化而降低。在非神经元神经外胚层肿瘤中，CD 24表达大多不存在。当存在时，它与间变性组织学特征的出现相关。逆转录-聚合酶链反应（RT-PCR）证明了一个独特的转录本相同的造血，发育和肿瘤神经组织的存在。RT-PCR和原位杂交技术表明，CD 24的表达是转录调控的。有趣的是，蛋白质印迹分析表明，根据组织（造血与神经），分化状态和神经母细胞瘤的起源可能反映糖基化程度的变化差异CD 24亚型。这表明涉及翻译后修饰的CD 24调节的额外水平。
CD24 is a glycoprotein with an unusual structure consisting of a small protein core extensively glycosylated and linked to the outer surface of the plasma membrane by a glycosylphosphatidylinositol (GPI) lipid anchor. Its murine homolog mCD24 is transiently expressed during the development and differentiation of the hematopoietic and neural cell lineages. We have searched for the expression of CD24 in the developing and in the mature human brain as well as in a wide range of neuroectodermal tumors. Neuroblastomas, a subgroup of tumors able to maturate from undifferentiated features towards mature ganglioneuromas, were more extensively studied. Immunohistochemical studies demonstrated that CD24 is transiently expressed by neurons during human brain development. In neuroectodermal tumors, CD24 is a marker of neuronal tumors. Furthermore, in neuroblastomas, CD24 expression decreases as tumors differentiate. In non-neuronal neuroectodermal tumors, CD24 expression is mostly absent. When present, it correlates with the emergence of anaplastic histological features. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated the presence of an unique transcript identical in both hematopoietic, developing and tumoral nervous tissue. RT-PCR and in situ hydridization techniques showed that CD24 expression is transcriptionally regulated. Interestingly, Western blot analysis demonstrated differential CD24 isoforms according to the tissue (hematopoietic versus nervous), the differentiation status, and the origin of neuroblastomas likely reflecting variations in the extent of glycosylation. This indicates an additional level of regulation of CD24 involving post-translational modifications.