Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal and Conjunctival Epithelium
Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal and Conjunctival Epithelium
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DOI：
10.1167/iovs.08-3046
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发表时间：
2009-05-01
影响因子：
4.4
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Yoshida, Yusuke;Ban, Yuriko;Kinoshita, Shigeru
目的。紧密连接跨膜蛋白Claudin亚型的结合是个体紧密连接链紧密度变化的最重要的决定因素之一。角膜上皮的屏障功能远强于结膜上皮。本研究对在体人角膜和结膜上皮细胞中Claudin基因的表达和细胞分布进行了研究。方法采用逆转录-聚合酶链式反应检测Claudin基因的表达。结果在人角膜上皮细胞中检测到claudin-1、-2、-3、-4、-7、-9和-14的转录本。在人结膜上皮细胞中发现了claudin-1、-2、-4、-7、-9、-10和-14的转录本。免疫组织化学方法发现Claudin-1、-4和-7定位于人角膜和结膜上皮细胞膜上。在人的结膜上皮中，Claudin-10在几个(但不是全部)顶端上皮细胞与杯状细胞的交界处被观察到。Claudin-10在结膜上皮中顶端上皮细胞和杯状细胞之间的几个交界处有明显的表达。除Claudin-10在结膜上皮细胞中的表达外，角膜和结膜上皮细胞中Claudin亚型的表达相似。因此，这两种上皮类型之间在Claudin亚型表达的特定比例或它们的磷酸化状态方面肯定是不同的。杯状细胞在结膜上皮中的分布也影响屏障功能的差异。(投资眼科VS科学。网址：10.1167/iovs.08-3046
PURPOSE. The combination of the tight junction transmembrane protein claudin subtypes is one of the most important determinants of variations in the tightness of individual paired tight junction strands. The barrier function of corneal epithelium is much stronger than that of conjunctival epithelium. In this study, the expression and cellular distribution of claudin species in in vivo human corneal and conjunctival epithelium were investigated.METHODS. Reverse transcription-polymerase chain reaction was used to reveal the claudin mRNA. Immunohistochemistry was used to determine the tissue distribution of tight junction-related proteins and MUC5AC.RESULTS. Transcripts for claudin- 1, -2, -3, -4, -7, -9, and -14 were identified in human corneal epithelium. Transcripts for claudin-1, -2, -4, -7, -9, -10, and -14 were identified in human conjunctival epithelium. By immunohistochemistry, claudin- 1, -4, and -7 were found to be localized at the membrane of human corneal and conjunctival epithelial cells. In human conjunctival epithelium, claudin- 10 staining was observed at several, but not all, apical epithelial cell-to-goblet cell junctions.CONCLUSIONS. Claudin-1, -4, and -7 are expressed in corneal and conjunctival epithelia. Claudin-10 is prominent at several junctions between apical epithelial cells and goblet cells in conjunctival epithelium. Except for claudin-10 expression in conjunctival epithelium, the claudin subtype expressions of corneal and conjunctival epithelia are similar. Therefore, there must be a difference between these two epithelial types with regard to the specific ratio of claudin subtypes expressed or their phosphorylation status. The distribution of goblet cells in conjunctival epithelium also influences the difference in barrier function. (Invest Ophthalmol Vis Sci. 2009;50:2103-2108) DOI:10.1167/iovs.08-3046