Identification of a novel vertebrate circadian clock-regulated gene encoding the protein nocturnin
Identification of a novel vertebrate circadian clock-regulated gene encoding the protein nocturnin
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DOI：
10.1073/pnas.93.25.14884
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发表时间：
1996-12-10
影响因子：
11.1
通讯作者：
Besharse, JC
中科院分区：
文献类型：
--
作者：
Green, CB;Besharse, JC
非洲爪蟾视网膜的光感受器是昼夜节律时钟的位置，是该系统中节律基因产物的差异显示屏幕的一部分，我们已经确定了在夜间表达高峰丰度的光感受器特异性mRNA， cDNA克隆显示一个开放阅读框，编码一个推测的355个氨基酸的蛋白质，我们将其命名为“夜因子”（nightturnin）。该蛋白与酵母转录因子CCR4的c端结构域以及亮氨酸拉链样二聚化基序具有很强的序列相似性，Nocturnin mRNA水平表现出高幅度的昼夜节律，核运行分析表明它在转录水平上受视网膜生物钟控制。我们的观察结果表明，夜曲素可能通过蛋白质-蛋白质相互作用发挥作用，要么作为生物钟的一个组成部分，要么作为生物钟功能的下游效应者。
Photoreceptors of the Xenopus laevis retina are the site of a circadian clock Ar part of a differential display screen for rhythmic gene products in this system, we have identified a photoreceptor-specific mRNA expressed in peak abundance at night, cDNA cloning revealed an open reading frame encoding a putative 355 amino acid protein that we have named ''nocturnin'' (for night-factor). This protein has strong sequence similarity to the C-terminal domain of the yeast transcription factor, CCR4, as well as a leucine zipper-like dimerization motif, Nocturnin mRNA levels exhibit a high amplitude circadian rhythm and nuclear run-on analysis indicates that it is controlled by the retinal circadian clock at the level of transcription, Our observations suggest that nocturnin may function through protein-protein interaction either as a component of the circadian clock or as a downstream effector of clock function.