Single-molecule conductance of double-stranded RNA oligonucleotides
Single-molecule conductance of double-stranded RNA oligonucleotides
复制标题
DOI：
10.1039/d1nr06925j
复制
发表时间：
2022-02-02
期刊：
影响因子：
6.7
通讯作者：
Vivancos, Juan M. Artes
中科院分区：
文献类型：
--
作者：
Chandra, Subrata;Arachchillage, Keshani G. Gunasinghe Pattiya;Vivancos, Juan M. Artes
RNA寡核苷酸对一系列生物功能和许多生物技术应用至关重要。在此，我们首次测量了单个双链RNA分子的电导，并将其与单个DNA: RNA杂交体的电导进行了比较。两种生物分子的平均电导值相似，但电导值的分布显示dsRNA的变异性要高一个数量级，这表明dsRNA具有更高的分子柔韧性。微秒分子动力学模拟解释了这种差异，并提供了结构上的见解，以更高的稳定性DNA: RNA双工与原子水平的细节。核糖环上的2 '-OH基团和RNA链上的碱基的旋转会削弱碱基堆叠相互作用，影响电荷传输，并使dsRNA的单分子电导更加可变（动态无序），从而破坏双相结构的稳定性。结果表明，最先进的生物分子电子学技术和计算方法的强大结合可以以前所未有的空间分辨率为生物分子的生物物理学提供有价值的见解。
RNA oligonucleotides are crucial for a range of biological functions and in many biotechnological applications. Herein, we measured, for the first time, the conductance of individual double-stranded (ds)RNA molecules and compared it with the conductance of single DNA : RNA hybrids. The average conductance values are similar for both biomolecules, but the distribution of conductance values shows an order of magnitude higher variability for dsRNA, indicating higher molecular flexibility of dsRNA. Microsecond Molecular Dynamics simulations explain this difference and provide structural insights into the higher stability of DNA : RNA duplex with atomic level of detail. The rotations of 2 '-OH groups of the ribose rings and the bases in RNA strands destabilize the duplex structure by weakening base stacking interactions, affecting charge transport, and making single-molecule conductance of dsRNA more variable (dynamic disorder). The results demonstrate that a powerful combination of state-of-the-art biomolecular electronics techniques and computational approaches can provide valuable insights into biomolecules' biophysics with unprecedented spatial resolution.