Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation.
Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation.
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1 型蛋白磷酸酶与 IpL1 蛋白激酶相反，调节酵母染色体分离。
DOI：
10.1128/mcb.14.7.4731-4740.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
Chan,CS
中科院分区：
文献类型：
--
作者：
Francisco,L;Wang,W;Chan,CS
IPL1基因是酿酒酵母高保真染色体分离所必需的，条件突变株在37℃时严重分离染色体，本文报道IPL1编码一种必需的蛋白激酶，其功能在每个细胞周期的后期是必需的。在26℃的允许生长温度下，ipl1突变细胞在从抗微管药物诺康唑引起的短暂的G2/M期停滞中恢复时出现缺陷。为了确定与IPLL蛋白激酶一起调节酵母染色体分离的其他基因产物，我们分离到了DIS2SHGLC7基因的一个截短版本，作为一种剂量依赖的纤维突变抑制因子。DIS2SHGLC7被预测编码1型蛋白磷酸酶的催化亚单位(PP1C)。全长DIS2SHGLC7基因的过表达导致野生型细胞中的染色体错误分离，并加剧突变表型inipl1细胞。此外，glc7-1突变可部分抑制其突变。这些结果表明，I型蛋白磷酸酶在体内与Ipl1蛋白激酶相反，以确保染色体分离的高保真。
TheIPL1gene is required for high-fidelity chromosome segregation in the budding yeastSaccharomyces cerevisiae.Conditionalipl1tsmutants missegregate chromosomes severely at 37°C. Here, we report thatIPL1encodes an essential putative protein kinase whose function is required during the later part of each cell cycle. At 26°C, the permissive growth temperature,ipl1mutant cells are defective in the recovery from a transient G2/M-phase arrest caused by the antimicrotubule drug nocodazole. In an effort to identify additional gene products that participate with the Ipll protein kinase in regulating chromosome segregation in yeast, a truncated version of the previously identifiedDIS2SHGLC7gene was isolated as a dosage-dependent suppressor ofipl1tsmutations.DIS2SHGLC7is predicted to encode a catalytic subunit (PP1C) of type 1 protein phosphatase. Overexpression of the full-lengthDIS2SHGLC7gene results in chromosome missegregation in wild-type cells and exacerbates the mutant phenotype inipl1cells. In addition, theglc7-1mutation can partially suppress theipl1-1mutation. These results suggest that type 1 protein phosphatase acts in opposition to the Ipl1 protein kinase in vivo to ensure the high fidelity of chromosome segregation.