Tumor-promoting phorbol esters promote melanogenesis and prevent expression of the adrenergic phenotype in quail neural crest cells.
Tumor-promoting phorbol esters promote melanogenesis and prevent expression of the adrenergic phenotype in quail neural crest cells.
复制标题
促肿瘤佛波酯促进黑色素生成并阻止鹌鹑神经嵴细胞中肾上腺素能表型的表达。
DOI：
10.1111/j.1432-0436.1981.tb01165.x
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Sieber,F
中科院分区：
文献类型：
--
作者：
Sieber-Blum,M;Sieber,F
促肿瘤佛波醇酯用于操纵神经嵴细胞的体外发育。当平板在克隆密度在第二次培养，鹌鹑神经嵴细胞从躯干区域引起了三种类型的集落，着色，无色素，和混合。着色集落仅由黑素细胞组成;高达50%的未着色和混合集落含有肾上腺素能神经细胞，可通过儿茶酚胺特异性组织荧光法进行鉴定。在培养基中添加有效的肿瘤促进剂缩短了神经嵴细胞的倍增时间，并改变了它们的形态学外观。它还延迟了色素沉着的发生，阻止了肾上腺素能表型的表达，减少了未色素沉着和混合集落的数量，并增加了色素沉着集落的数量，最有可能是通过引导祖细胞优先黑素生成途径。佛波醇酯促进小鼠皮肤肿瘤的能力与其干扰鹌鹑神经嵴细胞体外发育的能力之间存在明显的相关性。有效的促进剂12-0-十四烷酰基佛波醇13-乙酸酯（TPA）和佛波醇12，13-二癸酸酯（PDD）最有效，佛波醇12，13-二乙酸酯（PDA）的效果相当差，非促进类似物4-0-甲基12-0-十四烷酰基佛波醇13-乙酸酯（4 -0-methyl 12-0-tetradecanoyl phorbol 13-acetate）（4-0-Me-TPA）和4α-佛波醇12，13-二癸酸酯（4α-PDD）和母体醇佛波醇（PHR）几乎没有或没有影响。
Tumor‐promoting phorbol esters were used to manipulate the in vitro development of neural crest cells. When plated at clonal density in secondary culture, quail neural crest cells from the trunk region gave rise to three types of colonies, pigmented, unpigmented, and mixed. Pigmented colonies consisted exclusively of melanocytes; up to 50% of the unpigmented and mixed colonies contained adrenergic nerve cells which could be identified by a catecholamine‐specific histofluorescence method. Addition of potent tumor promoters to the culture medium shortened the doubling time of neural crest cells and altered their morphologic appearance. It also delayed the onset of pigmentation, prevented the expression of the adrenergic phenotype, reduced the number of unpigmented and mixed colonies, and increased the number of pigmented colonies, most likely by directing progenitor cells preferentially to the melanogenic pathway. There was a clear correlation between the ability of phorbol esters to promote skin tumors in mice and their ability to interfere with the in vitro development of quail neural crest cells. The potent promoters 12–0–tetradecanoyl phorbol 13–acetate (TPA) and phorbol 12,13–didecanoate (PDD) were most effective, phorbol 12,13–diacetate (PDA) was considerably less effective, the nonpromoting analogues 4–0–methyl 12–0–tetradecanoyl phorbol 13–acetate (4–0–Me‐TPA) and 4α‐phorbol 12,13–didecanoate (4α‐PDD) and the parent alcohol phorbol (PHR) had little or no effect.