DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication
DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication
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DOI：
10.1128/jvi.01517-07
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发表时间：
2007-12-01
影响因子：
5.4
通讯作者：
Kato, Nobuyuki
中科院分区：
文献类型：
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作者：
Ariumi, Yasuo;Kuroki, Misao;Kato, Nobuyuki
DDX3 是一种 DEAD-box RNA 解旋酶，可与丙型肝炎病毒 (HCV) 核心蛋白结合。然而，DDX3 在 HCV 复制中的作用仍不清楚。在此，我们证明，通过慢病毒载体转导，在表达针对 DDX3 的短发夹 RNA 的 HuH-7 衍生细胞中，基因组长度的 HCV RNA（HCV-O，基因型 1b）及其复制子 RNA 的积累均受到显着抑制。此外，在接种细胞培养物产生的 HCVcc 后，DDX3 敲低细胞中 JFH1（基因型 2a）的 RNA 复制和核心释放到培养物上清液中均受到抑制。因此，DDX3是HCV RNA复制所必需的。
DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.