The role of LXRα in goose primary hepatocyte lipogenesis
The role of LXRα in goose primary hepatocyte lipogenesis
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DOI：
10.1007/s11010-008-9937-8
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发表时间：
2009-02-01
影响因子：
4.3
通讯作者：
Zhang, Zhongxian
中科院分区：
文献类型：
--
作者：
Han, Chunchun;Wang, Jiwen;Zhang, Zhongxian
在这项研究中，我们研究了肝脏X受体α（LXR α）在鹅脂肪生成中的作用，以了解哺乳动物和水禽之间肝脏脂肪变性机制的差异。分离原代鹅肝细胞，并用LXR α激动剂T0901317处理。在原代肝细胞中测量甘油三酯（TG）蓄积、乙酰辅酶A羧化酶α（ACC α）和脂肪酸合成酶（FAS）活性以及LXR α、固醇调节元件结合蛋白-1（SREBP-1）、FAS、ACC α和脂蛋白脂肪酶（LPL）的基因表达水平。我们发现在To-901317存在下，TG积累、ACC和FAS活性以及LXR α、SREBP-1、FAS、ACC α和LPL基因的mRNA水平呈剂量依赖性上调。To-901317可诱导SREBP-1与ACC α SRE序列的结合（P < 0.05）。总之，在鹅原代肝细胞中，LXR α通过激活SREBP-1及其靶基因参与脂肪生成途径的诱导。
In this study, we investigate the role of liver X receptor alpha (LXR alpha) in lipogenesis in geese in order to understand the differences in hepatic steatosis mechanisms between mammals and waterfowl. Primary goose hepatocytes were isolated and treated with the LXR alpha agonist T0901317. Triglyceride (TG) accumulation, acetyl-CoA carboxylase alpha (ACC alpha) and fatty acid synthase (FAS) activities, and gene expression levels of LXR alpha, sterol regulatory element-binding proteins-1 (SREBP-1), FAS, ACC alpha and lipoprotein lipase (LPL) were measured in primary hepatocytes. We found a dose-dependent up-regulation of TG accumulation, ACC, and FAS activities and the mRNA levels of LXR alpha, SREBP-1, FAS, ACC alpha, and LPL genes in the presence of To-901317. We also found that binding of nuclear SREBP-1 to ACC alpha SRE sequence was induced by To-901317 (P < 0.05). In conclusion, LXR alpha is involved in the induction of the lipogenic pathway through activation of SREBP-1 and its target genes in goose primary hepatocytes.