Construction of a library of structurally diverse ribonucleopeptides with catalytic groups
Construction of a library of structurally diverse ribonucleopeptides with catalytic groups
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具有催化基团的结构多样化核糖核酸肽文库的构建
DOI：
10.1016/j.bmc.2017.02.007
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
T. Morii
中科院分区：
文献类型：
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作者：
T. Tamura;S. Nakano;E. Nakata;T. Morii
对由蛋白质或核酸组成的结构多样的文库进行功能筛选是获得具有独特分子识别特征的受体或适体的有效方法。然而，进一步修饰这些选定的受体以发挥新的所需功能仍然是一项具有挑战性的任务。我们已经构建了一个结构多样的核糖核肽（RNP）与催化基团，其中的催化基团与各种方向对齐对RNA亚基的ATP结合口袋修饰的库。作为原理验证，成功地进行了用于酯水解催化反应的构建的RNP文库的筛选。底物结合RNA文库和催化基团修饰肽文库的大小都是独立可扩展的，因此，RNP文库的大小可以通过这两个亚基的组合来扩大。我们预计，功能化和结构多样的RNP库将扩大各种其他催化反应。
Functional screening of structurally diverse libraries consisting of proteins or nucleic acids is an effective method to obtain receptors or aptamers with unique molecular recognition characteristics. However, further modification of these selected receptors to exert a newly desired function is still a challenging task. We have constructed a library of structurally diverse ribonucleopeptides (RNPs) that are modified with a catalytic group, in which the catalytic group aligns with various orientations against the ATP binding pocket of RNA subunit. As a proof-of-principle, the screening of the constructed RNP library for the catalytic reaction of ester hydrolysis was successfully carried out. The size of both the substrate-binding RNA library and the catalytic group modified peptide library are independently expandable, and thus, the size of RNPs library could be enlarged by a combination of these two subunits. We anticipate that the library of functionalized and structurally diverse RNPs would be expanded for various other catalytic reactions.