Co-localization and interaction of human organic anion transporter 4 with caveolin-1 in primary cultured human placental trophoblasts
Co-localization and interaction of human organic anion transporter 4 with caveolin-1 in primary cultured human placental trophoblasts
复制标题
原代培养的人胎盘滋养层细胞中人有机阴离子转运蛋白4与caveolin-1的共定位和相互作用
DOI：
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发表时间：
2008
影响因子：
12.8
通讯作者：
S. Cha
中科院分区：
文献类型：
--
作者：
W. Lee;J. Choi;S. Cha
人有机阴离子转运体4（hOAT4）已被确定为OAT家族的第四种异构体。hOAT4有助于在细胞与其细胞外环境之间转运几种带负电荷的有机化合物。hOAT4的功能特性和调节机制仍有待阐明。众所周知，小窝蛋白在调节具有某些生物学功能的蛋白质方面发挥作用。为了解决这个问题，我们研究了hOAT4和小窝蛋白 - 1之间的共定位和相互作用。在从胎盘分离的培养人胎盘滋养层细胞中观察到了hOAT4和小窝蛋白 - 1（mRNA和蛋白质表达）。使用原代培养的人滋养层细胞进行的免疫细胞化学共聚焦显微镜检查显示，hOAT4和小窝蛋白 - 1共定位在细胞质膜上。这一发现通过使用从滋养层细胞分离的富含小窝的膜组分和免疫沉淀物进行的蛋白质印迹分析得到了证实。当将hOAT4的合成互补RNA与非洲爪蟾小窝蛋白 - 1的乱序或反义寡脱氧核苷酸（ODN）共同注射到非洲爪蟾卵母细胞中时，反义ODN的共同注射显著降低了[³H]硫酸雌酮的摄取，而乱序ODN则没有。这些发现表明，hOAT4和小窝蛋白 - 1在细胞质膜上具有共同的细胞表达，并且在正常生理条件下，小窝蛋白 - 1上调hOAT4对有机阴离子化合物的摄取。
The human organic anion transporter 4 (hOAT4) has been identified as the fourth isoform of OAT family. hOAT4 contributes to move several negatively charged organic compounds between cells and their extracellular milieu. The functional characteristics and regulatory mechanisms of hOAT4 remain to be elucidated. It is well known that caveolin plays a role in modulating proteins having some biological functions. To address this issue, we investigated the co-localization and interaction between hOAT4 and caveolin-1. hOAT4 and caveolin-1 (mRNA and protein expression) were observed in cultured human placental trophoblasts isolated from placenta. The confocal microscopy of immuno-cytochemistry using primary cultured human trophoblasts showed hOAT4 and caveolin-1 were co-localized at the plasma membrane of the cell. This finding was confirmed by Western blot analysis using isolated caveolae-enriched membrane fractions and immune-precipitates from the trophoblasts. When synthesized cRNA of hOAT4 along with scrambled- or antisense-oligodeoxynucleotide (ODN) of Xenopus caveolin-1 were co-injected to Xenopus oocytes, the [3H]estrone sulfate uptake was significantly decreased by the co-injection of antisense ODN but not by scrambled ODN. These findings suggest that hOAT4 and caveolin-1 share a cellular expression in the plasma membrane and caveolin-1 up-regulates the organic anionic compound uptake by hOAT4 under the normal physiological condition.
影响因子：
2.9
作者：
Razani, B;Park, DS;Lisanti, MP
通讯作者：
Lisanti, MP
DOI：
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发表时间：
2000-08
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
V. Ganapathy;P. Prasad;M. E. Ganapathy;F. Leibach
通讯作者：
V. Ganapathy;P. Prasad;M. E. Ganapathy;F. Leibach