Rapid Surface Cooling by ThermoSuit System Dramatically Reduces Scar Size, Prevents Post-Infarction Adverse Left Ventricular Remodeling, and Improves Cardiac Function in Rats.
Rapid Surface Cooling by ThermoSuit System Dramatically Reduces Scar Size, Prevents Post-Infarction Adverse Left Ventricular Remodeling, and Improves Cardiac Function in Rats.
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DOI：
10.1161/jaha.115.002265
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发表时间：
2015-06-26
影响因子：
5.4
通讯作者：
Kloner RA
中科院分区：
文献类型：
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作者：
Dai W;Herring MJ;Hale SL;Kloner RA
在大鼠心肌梗死(MI)模型上，观察了无创性ThermoSuit瞬时低温对心肌梗死(MI)瘢痕大小、左心室(LV)重构及心功能的长期影响。将大鼠随机分为常温组和亚低温组(每组14只)，结扎冠状动脉30min，再灌流6周。低温治疗：大鼠冠状动脉阻断后2分钟放入ThermoSuit仪，待核心体温达到32°C(≈为8分钟)后取出，复温。恢复6周后，低温组大鼠的瘢痕面积明显缩小(以占左室面积的百分比表示：低温，6.5±1.1%；常温，19.4±1.7%；P=1.3×10−6)；左室前壁明显增厚(低温，1.5 7±0.09 mm；常温，1.0 7±0.0 5 mm；P=3.4×10−5)；死后左室容量减少(低温，0.45±0.0 4；常温，0.6±0.0 3；P=0.028)；超声心动图显示左室短轴缩短率改善(低温，37.2±2.8%；常温(18.9±2.3%；P=0.0002)和左心室声学造影左心室射血分数(低温组66.8±2.3%；常温组56.0±2.0%；P=0.0014)。在心肌梗塞急性期使用ThermoSuit设备进行快速、瞬时的无创表面冷却，可将瘢痕大小减少66.5%，减轻梗塞后不利的左心室扩张和重构，并改善实验性心肌梗塞慢性期的心功能。
The long-term effects of transient hypothermia by the non-invasive ThermoSuit apparatus on myocardial infarct (MI) scar size, left ventricular (LV) remodeling, and LV function were assessed in rat MI model. Rats were randomized to normothermic or hypothermic groups (n=14 in each group) and subjected to 30 minutes coronary artery occlusion and 6 weeks of reperfusion. For hypothermia therapy, rats were placed into the ThermoSuit apparatus at 2 minutes after the onset of coronary artery occlusion, were taken out of the apparatus when the core body temperature reached 32°C (in ≈8 minutes), and were then allowed to rewarm. After 6 weeks of recovery, rats treated with hypothermia demonstrated markedly reduced scar size (expressed as % of left ventricular area: hypothermia, 6.5±1.1%; normothermia, 19.4±1.7%; P=1.3×10−6); and thicker anterior LV wall (hypothermia, 1.57±0.09 mm; normothermia, 1.07±0.05 mm; P=3.4×10−5); decreased postmortem left ventricular volume (hypothermia, 0.45±0.04 mL; normothermia, 0.6±0.03 mL; P=0.028); and better LV fractional shortening by echocardiography (hypothermia, 37.2±2.8%; normothermia, 18.9±2.3%; P=0.0002) and LV ejection fraction by LV contrast ventriculography (hypothermia, 66.8±2.3%; normothermia, 56.0±2.0%; P=0.0014). Rapid, transient non-invasive surface cooling with the ThermoSuit apparatus in the acute phase of MI decreased scar size by 66.5%, attenuated adverse post-infarct left ventricular dilation and remodeling, and improved cardiac function in the chronic phase of experimental MI.