Mechanical instability and interfacial energy drive biofilm morphogenesis
Mechanical instability and interfacial energy drive biofilm morphogenesis
复制标题
DOI：
10.7554/elife.43920
复制
发表时间：
2019-03
期刊：
影响因子：
7.7
通讯作者：
Jing Yan;Chenyi Fei;Sheng Mao;Alexis Moreau;N. Wingreen;A. Košmrlj;H. Stone;B. Bassler
中科院分区：
文献类型：
--
作者：
Jing Yan;Chenyi Fei;Sheng Mao;Alexis Moreau;N. Wingreen;A. Košmrlj;H. Stone;B. Bassler
被称为生物膜的表面附着细菌群落显示出多样性的形态。虽然生物膜形成所需的结构和调节成分是已知的，但不了解这些基本成分如何促进生物膜表面形态。在这里，使用霍乱弧菌作为我们的模型系统，我们结合联合收割机的机械测量，理论和模拟，定量图像分析，表面能表征，和诱变显示，机械不稳定性，包括扭曲和分层，背后的形态生成程序的生长生物膜。我们还确定界面能作为机械形态的关键驱动力，因为它决定了新的生成和现有界面的湮灭。最后，我们发现了机械形态发生和生物膜扩张之间的反馈，从而形成了整体生物膜轮廓。我们在细菌生物膜中发现的依赖于机械不稳定性和界面能的形态发生原理应该普遍适用于高等生物组织中的形态发生过程。
Surface-attached bacterial communities called biofilms display a diversity of morphologies. Although structural and regulatory components required for biofilm formation are known, it is not understood how these essential constituents promote biofilm surface morphology. Here, using Vibrio cholerae as our model system, we combine mechanical measurements, theory and simulation, quantitative image analyses, surface energy characterizations, and mutagenesis to show that mechanical instabilities, including wrinkling and delamination, underlie the morphogenesis program of growing biofilms. We also identify interfacial energy as a key driving force for mechanomorphogenesis because it dictates the generation of new and the annihilation of existing interfaces. Finally, we discover feedback between mechanomorphogenesis and biofilm expansion, which shapes the overall biofilm contour. The morphogenesis principles that we discover in bacterial biofilms, which rely on mechanical instabilities and interfacial energies, should be generally applicable to morphogenesis processes in tissues in higher organisms.