The effect of culture media on large-scale expansion and characteristic of adipose tissue-derived mesenchymal stromal cells
The effect of culture media on large-scale expansion and characteristic of adipose tissue-derived mesenchymal stromal cells
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DOI：
10.1186/s13287-019-1331-9
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发表时间：
2019-08-05
影响因子：
7.5
通讯作者：
Cichon, Tomasz
中科院分区：
文献类型：
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作者：
Czapla, Justyna;Matuszczak, Sybilla;Cichon, Tomasz
脂肪组织来源的间充质间质细胞（ASCs）在再生医学中作为先进治疗药物（ATMP）显示出一些有前景的特性。然而，它们的来源、分离方法和扩增程序不同，导致实验室和临床结果难以比较。方法从抽脂液中分离ASCs，在三种不同的培养基中培养：α - MEM和DMEM作为基础培养基，添加10%的人血小板裂解液（hPL）， DMEM添加20%的胎牛血清（FBS）和bFGF作为金标准培养基。随后，我们评估了培养基对ASCs生长动力学、形态和免疫表型、分化能力、克隆潜能和分泌谱的影响。结果所有培养的ASCs具有相似的形态和相似的克隆潜能，并具有向脂肪细胞、成骨细胞和成软骨细胞三种细胞系分化的能力。所有培养的ASCs的免疫表型符合国际细胞治疗学会（ISCT）的指南，允许将细胞定义为间充质间质细胞（MSC） (>= 95% CD105, CD73， CD90和
Background Adipose tissue-derived mesenchymal stromal cells (ASCs) have been shown to exhibit some promising properties of their use in regenerative medicine as advanced therapy medicinal products (ATMP). However, different sources of their origin, methods of isolation, and expansion procedures cause the laboratory and clinical results difficult to compare. Methods ASCs were isolated from lipoaspirates and cultured in three different medium formulations: alpha MEM and DMEM as a basal medium supplemented with 10% of human platelet lysate (hPL) and DMEM supplemented with 20% fetal bovine serum (FBS) and bFGF as a gold standard medium. Subsequently, the impact of culture media on ASCs growth kinetics, their morphology and immunophenotype, ability to differentiate, clonogenic potential, and secretion profile was evaluated. Results All cultured ASCs lines showed similar morphology and similar clonogenic potential and have the ability to differentiate into three lines: adipocytes, osteoblasts, and chondroblasts. The immunophenotype of all cultured ASCs was consistent with the guidelines of the International Society for Cell Therapy (ISCT) allowing to define cells as mesenchymal stromal cell (MSC) (>= 95% CD105, CD73, CD90 and