BMP-3 mRNA expression during endochondral ossification of mouse bone tissue
BMP-3 mRNA expression during endochondral ossification of mouse bone tissue
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DOI：
10.1267/ahc.34.1
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发表时间：
2001-01-01
影响因子：
2.4
通讯作者：
Kitazawa, S
中科院分区：
文献类型：
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作者：
Fujimoto, T;Kitazawa, R;Kitazawa, S
骨形态发生蛋白 (BMP)-3/骨生成素具有多种生物学功能，从骨诱导到发育器官发生。为了阐明BMP-3在骨形成和维持过程中的作用，我们使用PCR产生的地高辛标记的单链DNA探针，通过原位杂交（ISH）研究了成年小鼠正常骨和骨折骨的4％多聚甲醛固定的脱钙骨以及小鼠胎儿全身中BMP-3 mRNA的表达。我们在电子显微镜水平上改进的原位杂交技术也被应用于鉴定发育中的胎儿骨的软骨内骨化过程中表达BMP-3的特定细胞类型。除了在发育中的胎儿支气管上皮和大脑胶质细胞中表达外，BMP-3的表达主要在正常生长板成熟过程中的软骨细胞以及成人长骨和发育中的胎儿编织骨的骨折愈伤组织中观察到，表明BMP-3表达与间充质细胞分化为软骨细胞谱系无关，而是与未成熟软骨细胞分化为更成熟的软骨细胞有关。软骨细胞。
Bone morphogenetic protein (BMP)-3/osteogenin has a diverse spectrum of biological functions ranging from bone induction to developmental organogenesis. To clarify the role of BMP-3 during bone formation and maintenance, we investigated the expression of BMP-3 mRNA by in situ hybridization (ISH) on 4% paraformaldehyde fixed decalcified bone of normal and fractured bone of adult mice and in the whole body of the mouse fetus with digoxigenin-labeled single-stranded DNA probes generated by PCR. Our modified in situ hybridization technique at the electron microscopic level was also applied to identify specific cell types expressing BMP-3 during endochondral ossification in the developing fetal bone. Besides its expression in the developing fetal bronchial epithelium and glial cells of the brain, BMP-3 expression was observed mainly on chondrocytic cells during maturation in the normal growth plate and the fractured callus of the adult long bone and the developing fetal woven bone, suggesting that BMP-3 expression was related not to differentiation of mesenchymal cells into the chondrocytic cell lineage but to the differentiation of immature chondrocytic cells into more mature chondrocytes.