Guanine nanowire based amplification strategy: Enzyme-free biosensing of nucleic acids and proteins
Guanine nanowire based amplification strategy: Enzyme-free biosensing of nucleic acids and proteins
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基于鸟嘌呤纳米线的扩增策略：核酸和蛋白质的无酶生物传感
DOI：
10.1016/j.bios.2015.11.070
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发表时间：
2016-04-15
影响因子：
12.6
通讯作者：
Li, Nian Bing
中科院分区：
文献类型：
--
作者：
Gao, Zhong Feng;Huang, Yan Li;Li, Nian Bing
核酸和蛋白质的灵敏和特异性检测在食品、法医筛查、临床和环境监测中起着至关重要的作用。信号放大技术在生物分子检测中的应用是一个巨大的挑战。在此，我们描述了一种新的信号放大策略的基础上形成的鸟嘌呤纳米线的核酸和蛋白质（凝血酶）在室温下的定量检测。在分析物和镁离子的存在下，鸟嘌呤纳米线可以在10分钟内形成。相比，广泛使用的单一G-四链体生物催化标记单元，在我们的测定的检测限提高了两个数量级。该方法避免了繁琐的化学标记过程、复杂的编程任务和复杂的设备，为构建选择性和灵敏的生物传感平台提供了理想的候选。(C)2015 Elsevier B. V.版权所有。
Sensitive and specific detection of nucleic acids and proteins plays a vital role in food, forensic screening, clinical and environmental monitoring. There remains a great challenge in the development of signal amplification method for biomolecules detection. Herein, we describe a novel signal amplification strategy based on the formation of guanine nanowire for quantitative detection of nucleic acids and proteins (thrombin) at room temperature. In the presence of analytes and magnesium ions, the guanine nanowire could be formed within 10 min. Compared to the widely used single G-quadruplex biocatalytic label unit, the detection limits are improved by two orders of magnitude in our assay. The proposed enzyme-free method avoids fussy chemical label-ling process, complex programming task, and sophisticated equipment, which might provide an ideal candidate for the fabrication of selective and sensitive biosensing platform. (C) 2015 Elsevier B.V. All rights reserved.