Alpha-glucosidase inhibitory and antioxidant activities of various extracts of aerial parts of Fagonia indica Burm. F.
Alpha-glucosidase inhibitory and antioxidant activities of various extracts of aerial parts of Fagonia indica Burm. F.
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DOI：
10.4314/tjpr.v18i4.16
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发表时间：
2019-04-01
影响因子：
0.6
通讯作者：
Qaisar, Muhammad Naeem
中科院分区：
文献类型：
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作者：
Atiq-ur-Rehman;Latif, Abida;Qaisar, Muhammad Naeem
目的：研究金毛榉的体外抗氧化和抗糖尿病潜力。 f.变种。方法：以氯仿、正己烷、甲醇、水为溶剂，采用冷浸法制备该植物地上部分提取物。通过标准方法测定各种提取物的总黄酮和多酚含量。分别使用 1, 1-二苯基-2-三硝苯肼 (DPPH) 和体外 α-葡萄糖苷酶（麦芽糖酶）抑制测定法测定提取物的抗氧化和抗糖尿病活性。 结果：初步结果表明提取物中存在多种植物化学物质。氯仿提取物的总黄酮含量（65.98 +/- 1.63 mg QE/g）和多酚（26.75 +/- 1.09 mg GAE/g）含量最高。该提取物还显示出最高的自由基清除能力 (64.74 +/- 1.43 %)，IC50 值为 34.18 +/- 5.57 mu g/mL，而甲醇提取物则发挥最高的 α-葡萄糖苷酶（麦芽糖酶）抑制活性 (45.22 +/- 0.46 %)，半最大浓度 (IC50) 为 220.4 +/- 0.41 mu g/mL。结论：Fagonia indica地上部分的提取物具有显着的抗糖尿病和抗氧化作用，从而证明了传统使用该植物治疗糖尿病的合理性。
Purpose: To investigate in vitro antioxidant and anti-diabetic potentials of Fagonia indica Burm. f. Var. indica in order to provide scientific evidence for its traditional use.Methods: Cold maceration method was employed for the preparation of extracts of aerial parts of the plant using chloroform, n-hexane, methanol and water as solvents. Total flavonoid and polyphenolic content of various extracts were determined by standard methods. The antioxidant and anti-diabetic activities of the extracts were determined using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and in vitro alpha-glucosidase (maltase) inhibitory assays, respectively.Results: Preliminary results indicated the presence of various phytochemicals in the extracts. The chloroform extract exhibited the highest contents of total flavonoids (65.98 +/- 1.63 mg QE/g) and polyphenols (26.75 +/- 1.09 mg GAE/g). This extract also showed the highest free radical scavenging (64.74 +/- 1.43 %) with IC50 value of 34.18 +/- 5.57 mu g/mL while the methanol extract exerted the highest alpha-glucosidase (maltase) inhibitory activity (45.22 +/- 0.46 %) with half-maximal concentration (IC50) of 220.4 +/- 0.41 mu g/mL.Conclusion: The extracts of the aerial parts of Fagonia indica possess significant anti-diabetic and antioxidant effect, thus justifying the traditional use of the plant for treatment of diabetes.